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PMID: 1987165 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The korF region of broad-host-range plasmid RK2 encodes two polypeptides with transcriptional repressor activity.

Journal of bacteriology ·Vol. 173 ·No. 2 ·1991-01-00 ·Pages 826-33

Jagura-Burdzy G, Ibbotson JP, Thomas CM

Abstract

Broad-host-range IncP plasmid RK2 possesses a series of operons involved in plasmid maintenance, whose expression is coordinated by a number of regulators, most of which are encoded in the central regulatory korA-korB operon. The nucleotide sequence of two new cistrons in this operon, comprising what we have previously designated the korF locus located between coordinates 57.0 and 56.0 kb on the genome of the IncP alpha plasmid RK2, is presented. The cistrons encode polypeptides of 173 and 175 amino acids. Each can repress transcription from the promoters for the kfrA (a monocistronic operon which follows the korA-korB operon) and trfA (a polycistronic operon encoding a putative single-stranded-DNA-binding protein as well as the essential plasmid replication protein TrfA) operons. In addition, the korF loci allow korB to repress kfrA transcription. Both polypeptides contain hydrophobic segments, suggesting that they may be membrane associated. KorFI is highly basic protein whose predicted properties are similar to those of histone like proteins.

Related Genes
korF
MeSH Terms
Amino Acid Sequence Base Sequence DNA, Bacterial/genetics Escherichia coli/genetics Molecular Sequence Data Operon Plasmids Protein Conformation Repressor Proteins/genetics Restriction Mapping Transcription, Genetic
Chemicals
DNA, Bacterial Repressor Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jagura-Burdzy G
Department of Biological Sciences, University of Birmingham, England.
Ibbotson J P
Thomas C M
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45 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.2.826-833.1991
Published
1991-01-00
Pages
826-33
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207077
Subset
IM
Databases
GENBANK
M36479, M63255, M63305, M63306, M64332, S69596, S69601, S69604, S69828, S69830, Y00448
External Links
PubMed source DOI: 10.1128/jb.173.2.826-833.1991 PMC Full Text View journal details
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