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PMID: 1988542 Published · ppublish English Journal Article

Lack of feedback inhibition of V kappa gene rearrangement by productively rearranged alleles.

The Journal of experimental medicine ·Vol. 173 ·No. 2 ·1991-02-01 ·Pages 409-15

Harada K, Yamagishi H

Abstract

Circular DNAs excised by immunoglobulin kappa chain gene rearrangements were cloned and characterized. 16 of 17 clones examined were double recombination products containing a V kappa-J kappa rearrangement (coding joint) as well as the reciprocal element (signal joint) of another V kappa-J kappa rearrangement. These products suggested multiple recombination, primary inversion, and secondary excision. In primary events, 5 of 16 translational reading frames were in-phase. Thus, V kappa gene rearrangement may not be inhibited by the presence of a productively rearranged allele. An unusually large trinucleotide (P) insertion forming a palindrome of 12 nucleotides was also observed in one of the coding joints.

Related Genes
V<down>K</down>
MeSH Terms
Alleles Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA, Circular/genetics Feedback Gene Rearrangement, B-Lymphocyte/genetics Immunoglobulin Joining Region/genetics Immunoglobulin Variable Region/genetics Mice Mice, Inbred BALB C Molecular Sequence Data Protein Biosynthesis
Chemicals
DNA, Circular Immunoglobulin Joining Region Immunoglobulin Variable Region
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Harada K
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
Yamagishi H
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29 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.173.2.409
Published
1991-02-01
Pages
409-15
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2118803
Subset
IM
Databases
GENBANK
54753, 54754, 54755, 54756, 54757, 54758, 54759, 54760, 54761, 54762, 54763, 54764, 54765, 54766, 54767, 54768, 54769, 54770, 54771
External Links
PubMed source DOI: 10.1084/jem.173.2.409 PMC Full Text View journal details
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