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PMID: 1990286 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

The URE2 gene product of Saccharomyces cerevisiae plays an important role in the cellular response to the nitrogen source and has homology to glutathione s-transferases.

Molecular and cellular biology ·Vol. 11 ·No. 2 ·1991-02-00 ·Pages 822-32

Coschigano PW, Magasanik B

Abstract

The URE2 gene of Saccharomyces cerevisiae has been cloned and sequenced. It encodes a predicted polypeptide of 354 amino acids with a molecular weight of 40,226. Deletion of the first 63 amino acids does not have any effect on the function of the protein. Studies with disruption alleles of the URE2 and GLN3 genes showed that both genes regulate GLN1 and GDH2, the structural genes for glutamine synthetase and NAD-linked glutamate dehydrogenase, respectively, at the transcriptional level, but expression of the regulatory genes does not appear to be regulated. Active URE2 gene product was required for the inactivation of glutamine synthetase upon addition of glutamine to cells growing with glutamate as the source of nitrogen. The predicted URE2 gene product has homology to glutathione S-transferases. The gene has been mapped to chromosome XIV, 5.9 map units from petX and 3.4 map units from kex2.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA, Fungal/genetics,isolation & purification Escherichia coli/genetics Fungal Proteins/genetics Genes, Fungal Genetic Complementation Test Genotype Glutathione Peroxidase Glutathione Transferase/genetics Humans Molecular Sequence Data Nitrogen/metabolism Plasmids Prions Restriction Mapping Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Sequence Homology, Nucleic Acid
Chemicals
DNA, Fungal Fungal Proteins Prions Saccharomyces cerevisiae Proteins Glutathione Peroxidase URE2 protein, S cerevisiae Glutathione Transferase Nitrogen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Coschigano P W
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Magasanik B
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.2.822-832.1991
Published
1991-02-00
Pages
822-32
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359734
Subset
IM
Grants
NIGMS NIH HHS · GM-07446 · United States
Databases
GENBANK
M35268, M64559, M64560, M64561, M64562, M64563, M64564, M64565, M64566, X59842
External Links
PubMed source DOI: 10.1128/mcb.11.2.822-832.1991 PMC Full Text View journal details
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