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PMID: 19946692 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Progranulin expression is upregulated after spinal contusion in mice.

Acta neuropathologica ·Vol. 119 ·No. 1 ·2010-01-00 ·Pages 123-33

Naphade SB, Kigerl KA, Jakeman LB, Kostyk SK, Popovich PG, Kuret J

Abstract

Progranulin (proepithelin) is a pleiotropic growth-factor associated with inflammation and wound repair in peripheral tissues. It also has been implicated in the response to acute traumatic brain injury as well as to chronic neurodegenerative diseases. To determine whether changes in progranulin expression also accompany acute spinal cord injury, C57BL/6 mice were subjected to mid-thoracic (T9 level) contusion spinal cord injury and analyzed by immunohistochemical and biochemical methods. Whereas spinal cord sections prepared from non-injured laminectomy control animals contained low basal levels of progranulin immunoreactivity in gray matter, sections from injured animals contained intense immunoreactivity throughout the injury epicenter that peaked 7-14 days post injury. Progranulin immunoreactivity colocalized with myeloid cell markers CD11b and CD68, indicating that expression increased primarily in activated microglia and macrophages. Immunoblot analysis confirmed that progranulin protein levels rose after injury. On the basis of quantitative polymerase chain reaction analysis, increased protein levels resulted from a tenfold rise in progranulin transcripts. These data demonstrate that progranulin is dramatically induced in myeloid cells after experimental spinal cord injury and is positioned appropriately both spatially and temporally to influence recovery after injury.

MeSH Terms
Animals Antigens, CD/metabolism Antigens, Differentiation, Myelomonocytic/metabolism CD11b Antigen/metabolism Female Granulins Intercellular Signaling Peptides and Proteins/metabolism Macrophages/metabolism Mice Mice, Inbred C57BL Microglia/metabolism Nerve Fibers, Unmyelinated/metabolism Neuroimmunomodulation Progranulins Spinal Cord/metabolism Spinal Cord Injuries/metabolism Thoracic Vertebrae Time Factors Up-Regulation
Chemicals
Antigens, CD Antigens, Differentiation, Myelomonocytic CD11b Antigen CD68 protein, mouse Granulins Grn protein, mouse Intercellular Signaling Peptides and Proteins Progranulins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Naphade Swati B
Department of Molecular and Cellular Biochemistry, The Ohio State University College of Medicine, Columbus, OH 43210, USA.
Kigerl Kristina A
Jakeman Lyn B
Kostyk Sandra K
Popovich Phillip G
Kuret Jeff
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Article Info
Journal
Acta neuropathologica
Abbr.
Acta Neuropathol
ISSN
1432-0533
DOI
10.1007/s00401-009-0616-y
Published
2010-01-00
Epub
2009-00-28
Pages
123-33
Language
English
Region
Germany
NLM ID
0412041
PMCID
PMC4290888
Subset
IM
Grants
NINDS NIH HHS · P30 NS045758 · United States
NIA NIH HHS · R01 AG014452 · United States
NINDS NIH HHS · R01 NS043246 · United States
NINDS NIH HHS · NS045758 · United States
Corrections
CommentIn
PMID: 20012633
External Links
PubMed source DOI: 10.1007/s00401-009-0616-y PMC Full Text View journal details
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