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PMID: 1995944 Published · ppublish English Journal Article

Induction of human immunodeficiency virus type 1 expression in chronically infected cells is associated primarily with a shift in RNA splicing patterns.

Journal of virology ·Vol. 65 ·No. 3 ·1991-03-00 ·Pages 1291-303

Michael NL, Morrow P, Mosca J, Vahey M, Burke DS, Redfield RR

Abstract

We have analyzed the kinetics of human immunodeficiency virus type 1 (HIV-1) RNA induction in chronically infected T cells and promonocytes. A substantial amount of spliced mRNAs and assembled virions was found in resting cells. Induction increased the steady-state level of total HIV-1 RNA by 4-fold but increased the level of unspliced transcripts by 25-fold. This increase in unspliced RNA was reflected in the amount of virus seen by electron microscopy. These data suggest a mechanism for the induction of HIV-1 RNA in chronically infected cells involving a shift in splicing greatly favoring the stability of unspliced viral RNA with only a modest increase in total viral RNA. Analysis of the relative abundance of transcript classes is critical to the measurement of HIV-1 viral replication kinetics.

MeSH Terms
Base Sequence Cell Line Cell Transformation, Viral HIV-1/genetics,ultrastructure Humans Kinetics Microscopy, Electron Molecular Sequence Data Oligonucleotide Probes Polymerase Chain Reaction RNA Splicing RNA, Messenger/genetics RNA, Viral/biosynthesis,genetics,ultrastructure T-Lymphocytes Transcription, Genetic Virion/genetics
Chemicals
Oligonucleotide Probes RNA, Messenger RNA, Viral
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Michael N L
Department of Retroviral Research, Walter Reed Army Institute of Research, Rockville, Maryland 20850.
Morrow P
Mosca J
Vahey M
Burke D S
Redfield R R
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.3.1291-1303.1991
Published
1991-03-00
Pages
1291-303
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC239904
Subset
IM
Corrections
ErratumIn
PMID: 1942260
External Links
PubMed source DOI: 10.1128/JVI.65.3.1291-1303.1991 PMC Full Text View journal details
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