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PMID: 2005893 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Point mutations upstream of the yeast ADH2 poly(A) site significantly reduce the efficiency of 3'-end formation.

Molecular and cellular biology ·Vol. 11 ·No. 4 ·1991-04-00 ·Pages 2004-12

Hyman LE, Seiler SH, Whoriskey J, Moore CL

Abstract

The sequences directing formation of mRNA 3' ends in Saccharomyces cerevisiae are not well defined. This is in contrast to the situation in higher eukaryotes in which the sequence AAUAAA is known to be crucial to proper 3'-end formation. The AAUAAA hexanucleotide is found upstream of the poly(A) site in some but not all yeast genes. One of these is the gene coding for alcohol dehydrogenase, ADH2. Deletion or a double point mutation of the AAUAAA has only a small effect on the efficiency of the reaction, and in contrast to the mammalian system, it is most likely not operating as a major processing signal in the yeast cell. However, we isolated point mutations which reveal that a region located approximately 80 nucleotides upstream of the poly(A) site plays a critical role in either transcription termination, polyadenylation, or both. These mutations represent the first point mutations in yeasts which significantly reduce the efficiency of 3'-end formation.

Related Genes
ADH2
MeSH Terms
Alcohol Dehydrogenase/genetics Base Sequence Cloning, Molecular Genes, Fungal Molecular Sequence Data Mutation Poly A/genetics RNA, Fungal/metabolism RNA, Messenger/metabolism Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics
Chemicals
RNA, Fungal RNA, Messenger Poly A Alcohol Dehydrogenase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hyman L E
Department of Molecular Biology and Microbiology, Tufts University, Boston, Massachusetts 02111-1800.
Seiler S H
Whoriskey J
Moore C L
References (48)
48 references, click to expand
  1. Transcription terminator-like element within a Saccharomyces cerevisiae promoter region.
    Mol Cell Biol. 1986 Apr;6(4):1095-101 PMID: 3023868
  2. Definition of essential sequences and functional equivalence of elements downstream of the adenovirus E2A and the early simian virus 40 polyadenylation sites.
    Mol Cell Biol. 1985 Nov;5(11):2975-83 PMID: 3018490
  3. A trans-acting suppressor restores splicing of a yeast intron with a branch point mutation.
    Genes Dev. 1987 Jul;1(5):445-55 PMID: 2890553
  4. Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis.
    Nucleic Acids Res. 1987 Dec 10;15(23):9627-40 PMID: 3697078
  5. UASs and enhancers: common mechanism of transcriptional activation in yeast and mammals.
    Cell. 1988 Feb 12;52(3):303-5 PMID: 2894251
  6. Separation and characterization of a poly(A) polymerase and a cleavage/specificity factor required for pre-mRNA polyadenylation.
    Cell. 1988 Mar 11;52(5):731-42 PMID: 2830992
  7. Polyadenylation of mRNA precursors.
    Biochim Biophys Acta. 1988 May 6;950(1):1-12 PMID: 2896017
  8. The Saccharomyces cerevisiae ACP2 gene encodes an essential HMG1-like protein.
    Mol Cell Biol. 1988 Mar;8(3):1282-9 PMID: 2835668
  9. Multiple factors are required for specific RNA cleavage at a poly(A) addition site.
    Genes Dev. 1988 May;2(5):578-87 PMID: 2838381
  10. Multiple factors are required for poly(A) addition to a mRNA 3' end.
    Genes Dev. 1988 May;2(5):588-97 PMID: 3384332
  11. Transcription interferes with elements important for chromosome maintenance in Saccharomyces cerevisiae.
    Mol Cell Biol. 1988 May;8(5):2184-94 PMID: 3290652
  12. Orientation-dependent function of a short CYC1 DNA fragment in directing mRNA 3' end formation in yeast.
    Proc Natl Acad Sci U S A. 1988 Jul;85(14):5041-5 PMID: 2839828
  13. 3' cleavage and polyadenylation of mRNA precursors in vitro requires a poly(A) polymerase, a cleavage factor, and a snRNP.
    Cell. 1988 Sep 9;54(6):875-89 PMID: 2842067
  14. Splice site selection dominates over poly(A) site choice in RNA production from complex adenovirus transcription units.
    EMBO J. 1988 Jul;7(7):2107-16 PMID: 3416835
  15. RNA processing generates the mature 3' end of yeast CYC1 messenger RNA in vitro.
    Science. 1988 Dec 2;242(4883):1270-4 PMID: 2848317
  16. Impairment of yeast pre-mRNA splicing by potential secondary structure-forming sequences near the conserved branchpoint sequence.
    Nucleic Acids Res. 1988 Nov 25;16(22):10413-23 PMID: 2905037
  17. Competition between splicing and polyadenylation reactions determines which adenovirus region E3 mRNAs are synthesized.
    Mol Cell Biol. 1988 Aug;8(8):3291-7 PMID: 2463473
  18. Poly(A) polymerase purified from HeLa cell nuclear extract is required for both cleavage and polyadenylation of pre-mRNA in vitro.
    Mol Cell Biol. 1989 Jan;9(1):193-203 PMID: 2538718
  19. Mutational analysis of a yeast transcriptional terminator.
    Proc Natl Acad Sci U S A. 1989 Jun;86(11):4097-101 PMID: 2657739
  20. How RNA polymerase II terminates transcription in higher eukaryotes.
    Trends Biochem Sci. 1989 Mar;14(3):105-10 PMID: 2658217
  21. Some of the signals for 3'-end formation in transcription of the Saccharomyces cerevisiae Ty-D15 element are immediately downstream of the initiation site.
    Mol Cell Biol. 1989 Jun;9(6):2431-44 PMID: 2548082
  22. Definition of an efficient synthetic poly(A) site.
    Genes Dev. 1989 Jul;3(7):1019-25 PMID: 2570734
  23. Overproduction of yeast viruslike particles by strains deficient in a mitochondrial nuclease.
    Mol Cell Biol. 1989 Aug;9(8):3323-31 PMID: 2552292
  24. Transcription terminates near the poly(A) site in the CYC1 gene of the yeast Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1989 Nov;86(21):8348-52 PMID: 2554310
  25. Efficiency of utilization of the simian virus 40 late polyadenylation site: effects of upstream sequences.
    Mol Cell Biol. 1989 Oct;9(10):4248-58 PMID: 2573828
  26. Sequences upstream of AAUAAA influence poly(A) site selection in a complex transcription unit.
    Mol Cell Biol. 1989 Nov;9(11):4951-61 PMID: 2601703
  27. Nuclear pre-mRNA splicing in yeast.
    Yeast. 1989 Nov-Dec;5(6):439-57 PMID: 2694677
  28. A putative ATP binding protein influences the fidelity of branchpoint recognition in yeast splicing.
    Cell. 1990 Mar 9;60(5):705-17 PMID: 2138057
  29. RNA processing in vitro produces mature 3' ends of a variety of Saccharomyces cerevisiae mRNAs.
    Mol Cell Biol. 1990 Jun;10(6):2599-605 PMID: 2160581
  30. Sequences 5' to the polyadenylation signal mediate differential poly(A) site use in hepatitis B viruses.
    Genes Dev. 1990 May;4(5):764-76 PMID: 2379828
  31. Signal sequence for generation of mRNA 3' end in the Saccharomyces cerevisiae GAL7 gene.
    EMBO J. 1990 Nov;9(11):3691-7 PMID: 2209557
  32. Deletion analysis of the polyadenylation signal of a pea ribulose-1,5-bisphosphate carboxylase small-subunit gene.
    Plant Mol Biol. 1989 Aug;13(2):125-38 PMID: 2577506
  33. Upstream sequences other than AAUAAA are required for efficient messenger RNA 3'-end formation in plants.
    Plant Cell. 1990 Dec;2(12):1261-72 PMID: 1983794
  34. Hydroxylamine mutagenesis of HSV DNA and DNA fragments: introduction of mutations into selected regions of the viral genome.
    Virology. 1979 Oct 15;98(1):168-81 PMID: 225860
  35. Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information.
    Nucleic Acids Res. 1981 Jan 10;9(1):133-48 PMID: 6163133
  36. DNA sequence required for efficient transcription termination in yeast.
    Cell. 1982 Mar;28(3):563-73 PMID: 6280875
  37. Nucleotide sequence of the yeast alcohol dehydrogenase II gene.
    J Biol Chem. 1983 Feb 25;258(4):2674-82 PMID: 6337160
  38. Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast.
    Proc Natl Acad Sci U S A. 1983 Jul;80(14):4403-7 PMID: 6308621
  39. Evidence for the biochemical role of an internal sequence in yeast nuclear mRNA introns: implications for U1 RNA and metazoan mRNA splicing.
    Cell. 1983 Sep;34(2):395-403 PMID: 6616616
  40. The terminal RNA stem-loop structure and 80 bp of spacer DNA are required for the formation of 3' termini of sea urchin H2A mRNA.
    Cell. 1983 Dec;35(2 Pt 1):433-40 PMID: 6317188
  41. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  42. Sequences responsible for transcription termination on a gene segment in Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Aug;4(8):1515-20 PMID: 6436686
  43. Role of the conserved AAUAAA sequence: four AAUAAA point mutants prevent messenger RNA 3' end formation.
    Science. 1984 Nov 30;226(4678):1045-51 PMID: 6208611
  44. Transcription termination within the E1A gene of adenovirus induced by insertion of the mouse beta-major globin terminator element.
    Cell. 1985 Apr;40(4):897-905 PMID: 2580640
  45. Size and position of intervening sequences are critical for the splicing efficiency of pre-mRNA in the yeast Saccharomyces cerevisiae.
    Nucleic Acids Res. 1985 Jun 11;13(11):3791-804 PMID: 3892483
  46. Signals for transcription initiation and termination in the Saccharomyces cerevisiae plasmid 2 micron circle.
    Mol Cell Biol. 1985 Oct;5(10):2770-80 PMID: 3915534
  47. Transcription termination and the regulation of gene expression.
    Annu Rev Biochem. 1986;55:339-72 PMID: 3527045
  48. Alpha-thalassaemia caused by a poly(A) site mutation reveals that transcriptional termination is linked to 3' end processing in the human alpha 2 globin gene.
    EMBO J. 1986 Nov;5(11):2915-22 PMID: 3024968
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.4.2004-2012.1991
Published
1991-04-00
Pages
2004-12
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359886
Subset
IM
Grants
NIGMS NIH HHS · GM 12818-01A1 · United States
NIGMS NIH HHS · GM 41752-02 · United States
External Links
PubMed source DOI: 10.1128/mcb.11.4.2004-2012.1991 PMC Full Text View journal details
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