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PMID: 3892483 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Size and position of intervening sequences are critical for the splicing efficiency of pre-mRNA in the yeast Saccharomyces cerevisiae.

Nucleic acids research ·Vol. 13 ·No. 11 ·1985-06-11 ·Pages 3791-804

Klinz FJ, Gallwitz D

Abstract

The size of the 309 bp actin gene intron of the yeast Saccharomyces cerevisiae was enlarged by inserting DNA fragments of different lengths and sequence. Enlarging the intron above 551 bp, the largest known yeast intron, led to a decrease in splicing efficiency. The effect on transcript splicing was dependent on the length of the inserted fragments rather than their sequence. It was also observed that insertion of the actin gene intron into different regions of the normally unsplit yeast YP2 gene, significantly influenced the efficiency of splicing of the resulting transcripts. The splicing efficiency of splicing of with the increase of the distance between the mRNA cap site and the intervening sequence.

MeSH Terms
Actins/genetics Base Sequence Genes Genes, Fungal Nucleic Acid Hybridization Nucleic Acid Precursors/genetics Plasmids RNA Precursors RNA Splicing RNA, Messenger/genetics Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
Actins Nucleic Acid Precursors RNA Precursors RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Klinz F J
Gallwitz D
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.11.3791
Published
1985-06-11
Pages
3791-804
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341278
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/13.11.3791 PMC Full Text View journal details
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