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PMID: 200921 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Identification of amber and ochre mutants of the human virus Ad2+ND1.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 10 ·1977-10-00 ·Pages 4567-71

Gesteland RF, Wills N, Lewis JB, Grodzicker T

Abstract

Although human adenoviruses grow poorly in monkey cells, this defect can be overcome either by coinfection of cells with simian virus 40 (SV40) or by insertion of the relevant portion of the SV40 genome into the adenovirus genome to form an adenovirus-SV40 hybrid virus. The nondefective adenovirus-2-SV40 hybrid virus, Ad2+ND1, contains an insertion of 17% of the SV40 genome, which codes for at least part of a 30,000 dalton protein. A set of Ad2+ND1 host-range mutants that have lost the ability to grow in monkey cells and behave as point mutants fail to synthesize the 30,000 dalton ND1 protein. Translation in vitro of SV40-specific mRNA from mutant-infected cells produces unique short polypeptides instead of the 30,000 dalton protein. Here we show that this set of host-range mutants includes both ochre and amber nonsense mutations. When the SV40-specific mRNA from the host-range mutants is translated in vitro to produce the polypeptide fragments, yeast suppressor tRNA can partially restore synthesis of wild-type-size 30,000 dalton protein. By this assay, one mutant is ochre and two are amber.

MeSH Terms
Adenoviridae/genetics Cell-Free System DNA, Viral Genes, Viral Humans Mutation Nucleic Acid Hybridization Protein Biosynthesis RNA, Transfer/physiology RNA, Viral Simian virus 40 Viral Proteins/biosynthesis Yeasts/genetics
Chemicals
DNA, Viral RNA, Viral Viral Proteins RNA, Transfer
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gesteland R F
Wills N
Lewis J B
Grodzicker T
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44 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.10.4567
Published
1977-10-00
Pages
4567-71
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431987
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.10.4567 PMC Full Text View journal details
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