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PMID: 2020548 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The transcription factor LF-A1 interacts with a bipartite recognition sequence in the promoter regions of several liver-specific genes.

Nucleic acids research ·Vol. 19 ·No. 5 ·1991-03-11 ·Pages 1139-46

Ramji DP, Tadros MH, Hardon EM, Cortese R

Abstract

The transcription factors LF-A1 and LF-B1 are required for the cell-specific expression of the human alpha 1-antitrypsin gene in hepatocytes. We report here the purification and preliminary characterization of LF-A1. This protein, purified to homogeneity from calf liver nuclei by site-specific DNA affinity chromatography and reverse-phase HPLC, has a molecular mass of 40 kDa. Binding sites of LF-A1 are present in the promoter regions of several genes expressed in the liver (alpha 1-antitrypsin, apolipoproteins A1, B1, A4 and pyruvate kinase). Interestingly, the binding site of LF-A1 is bipartite and consists of two short sequence motifs (consensus: TGGACT/CT/C and TGGCCC) separated by a variable 'spacer' region. Insertion or deletion of 1-4 nucleotides in the 'spacer' region of the site in the alpha 1-antitrypsin promoter does not abolish DNA binding.

Related Genes
&agr 1AT APOA1 APOA4h APOA4r APOAII APOB1 PK hp hpr
MeSH Terms
Animals Base Sequence Binding Sites Cattle Chromatography, Affinity Chromatography, High Pressure Liquid DNA DNA-Binding Proteins Hepatocyte Nuclear Factor 1 Hepatocyte Nuclear Factor 1-alpha Hepatocyte Nuclear Factor 1-beta Liver/metabolism Molecular Sequence Data Nuclear Proteins Promoter Regions, Genetic Transcription Factors/isolation & purification,metabolism
Chemicals
DNA-Binding Proteins Hepatocyte Nuclear Factor 1-alpha Nuclear Proteins Transcription Factors Hepatocyte Nuclear Factor 1 Hepatocyte Nuclear Factor 1-beta DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ramji D P
European Molecular Biology Laboratory, Heidelberg, FRG.
Tadros M H
Hardon E M
Cortese R
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.5.1139
Published
1991-03-11
Pages
1139-46
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333792
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.5.1139 PMC Full Text View journal details
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