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PMID: 2027749 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification and analysis of antisense RNA target regions of the human immunodeficiency virus type 1.

Nucleic acids research ·Vol. 19 ·No. 7 ·1991-04-11 ·Pages 1421-6

Rittner K, Sczakiel G

Abstract

Antisense RNA, transcribed intracellularly from constitutive expression cassettes, inhibits the replication of the human immunodeficiency virus type 1 (HIV-1) as demonstrated by a quantitative microinjection assay in human SW480 cells. Infectious proviral HIV-1 DNA was co-microinjected together with a fivefold molar excess of plasmids expressing antisense RNA complementary to a set of ten different HIV-1 target regions. The most inhibitory antisense RNA expression plasmids were targeted against a 1 kb region within the gag open reading frame and against a 562 base region containing the coding sequences for the regulatory viral proteins tat and rev. Experimental evidence is presented that the antisense principle is the inhibitory mechanism in this assay system.

MeSH Terms
Cell Line Cloning, Molecular HIV Antigens/analysis HIV-1/genetics,physiology Humans Microinjections Plasmids RNA, Antisense/genetics RNA, Viral/genetics Substrate Specificity Virus Replication
Chemicals
HIV Antigens RNA, Antisense RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rittner K
Institut für Virusforschung, Deutsches Krebsforschungszentrum, Heidelberg, FRG.
Sczakiel G
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.7.1421
Published
1991-04-11
Pages
1421-6
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333895
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.7.1421 PMC Full Text View journal details
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