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PMID: 20308321 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

SWR1 complex poises heterochromatin boundaries for antisilencing activity propagation.

Molecular and cellular biology ·Vol. 30 ·No. 10 ·2010-05-00 ·Pages 2391-400

Zhou BO, Wang SS, Xu LX, Meng FL, Xuan YJ, Duan YM, Wang JY, Hu H, Dong X, Ding J, Zhou JQ

Abstract

In eukaryotes, chromosomal processes are usually modulated through chromatin-modifying complexes that are dynamically targeted to specific regions of chromatin. In this study, we show that the chromatin-remodeling complex SWR1 (SWR1-C) uses a distinct strategy to regulate heterochromatin spreading. Swr1 binds in a stable manner near heterochromatin to prepare specific chromosomal regions for H2A.Z deposition, which can be triggered by NuA4-mediated acetylation of histone H4. We also demonstrate through experiments with Swc4, a module shared by NuA4 and SWR1-C, that the coupled actions of NuA4 and SWR1-C lead to the efficient incorporation of H2A.Z into chromatin and thereby synergize heterochromatin boundary activity. Our results support a model where SWR1-C resides at the heterochromatin boundary to maintain and amplify antisilencing activity of histone H4 acetylation through incorporating H2A.Z into chromatin.

MeSH Terms
Adenosine Triphosphatases/genetics,metabolism Gene Expression Regulation, Fungal Gene Silencing Heterochromatin/metabolism Histones/genetics,metabolism Models, Genetic Protein Subunits/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism
Chemicals
Heterochromatin Histones Protein Subunits Saccharomyces cerevisiae Proteins Adenosine Triphosphatases Swr1 protein, S cerevisiae
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Zhou Bo O
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, 320 Yue-Yang Road, Shanghai 200031, China.
Wang Shan-Shan
Xu Lu-Xia
Meng Fei-Long
Xuan Yao-Ji
Duan Yi-Min
Wang Jian-Yong
Hu Hao
Dong Xianchi
Ding Jianping
Zhou Jin-Qiu
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
1098-5549
DOI
10.1128/MCB.01106-09
Published
2010-05-00
Epub
2010-00-22
Pages
2391-400
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC2863710
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.01106-09 PMC Full Text View journal details
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