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PMID: 14504221 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A targeted histone acetyltransferase can create a sizable region of hyperacetylated chromatin and counteract the propagation of transcriptionally silent chromatin.

Genetics ·Vol. 165 ·No. 1 ·2003-09-00 ·Pages 115-25

Chiu YH, Yu Q, Sandmeier JJ, Bi X

Abstract

Transcriptionally silent chromatin is associated with reduced histone acetylation and its propagation depends on histone hypoacetylation promoted by histone deacetylases. We show that tethered histone acetyltransferase (HAT) Esa1p or Gcn5p creates a segment of hyperacetylated chromatin that is at least 2.6 kb in size and counteracts transcriptional silencing that emanates from a silencer in yeast. Esa1p and Gcn5p counteract URA3 silencing even when they are targeted 1.7 kb downstream of the promoter and >2.0 kb from the silencer. The anti-silencing effect of a targeted HAT is strengthened by increasing the number of targeting sites, but impaired by events that enhance silencing. A tethered HAT can also counteract telomeric silencing. The anti-silencing effect of Gcn5p is abolished by a mutation that eliminated its HAT activity or by deleting the ADA2 gene encoding a structural component of Gcn5p-containing HAT complexes. These results demonstrate that a tethered HAT complex can create a sizable region of histone hyperacetylation and serve as a barrier to encroaching repressive chromatin.

MeSH Terms
Acetylation Acetyltransferases/physiology Chromatin/genetics,physiology Gene Expression Regulation, Fungal Gene Silencing Genes, Reporter Histone Acetyltransferases Yeasts/enzymology,genetics
Chemicals
Chromatin Acetyltransferases Histone Acetyltransferases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chiu Ya-Hui
Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA.
Yu Qun
Sandmeier Joseph J
Bi Xin
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/165.1.115
Published
2003-09-00
Pages
115-25
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1462738
Subset
IM
Grants
NIGMS NIH HHS · GM 62484 · United States
External Links
PubMed source DOI: 10.1093/genetics/165.1.115 PMC Full Text View journal details
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