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PMID: 2041733 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and characterization of maize cDNAs encoding a high mobility group protein displaying a HMG-box.

Nucleic acids research ·Vol. 19 ·No. 10 ·1991-05-25 ·Pages 2573-7

Grasser KD, Feix G

Abstract

cDNAs encoding a nonhistone chromosomal high mobility group (HMG) protein corresponding to the animal HMG1 family were isolated from a maize cDNA library using an immunoscreening approach. The cDNAs revealed an open reading frame of 471 base pairs together with 413 base pairs of flanking region, in agreement with the size of mRNA detected by Northern analysis of maize endosperm RNA. Like its animal counterparts the 17146 Da maize HMG protein contains a basic aminoterminus and an acidic carboxyterminus. The HMG-box region of this plant HMG protein shows striking sequence similarity to members of the vertebrate HMG1 family. Based on Southern blot hybridization analysis of genomic DNA, the isolated cDNA appears to be derived from a single or low copy gene.

MeSH Terms
Amino Acid Sequence Amino Acids/analysis Animals Base Sequence Blotting, Northern Blotting, Southern DNA/isolation & purification High Mobility Group Proteins/genetics Humans Molecular Sequence Data Multigene Family Open Reading Frames Restriction Mapping Sequence Homology, Nucleic Acid Zea mays/genetics
Chemicals
Amino Acids High Mobility Group Proteins DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Grasser K D
Institut für Biologie III, Freiburg, FRG.
Feix G
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43 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.10.2573
Published
1991-05-25
Pages
2573-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328172
Subset
IM
Databases
GENBANK
X15932, X15933, X15934, X15935, X54475, X54476, X54477, X54478, X54479, X58282
External Links
PubMed source DOI: 10.1093/nar/19.10.2573 PMC Full Text View journal details
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