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PMID: 20566706 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously.

Plant physiology ·Vol. 153 ·No. 4 ·2010-08-00 ·Pages 1747-58

Wei X, Xu J, Guo H, Jiang L, Chen S, Yu C, Zhou Z, Hu P, Zhai H, Wan J

Abstract

The three most important agronomic traits of rice (Oryza sativa), yield, plant height, and flowering time, are controlled by many quantitative trait loci (QTLs). In this study, a newly identified QTL, DTH8 (QTL for days to heading on chromosome 8), was found to regulate these three traits in rice. Map-based cloning reveals that DTH8 encodes a putative HAP3 subunit of the CCAAT-box-binding transcription factor and the complementary experiment increased significantly days to heading, plant height, and number of grains per panicle in CSSL61 (a chromosome segment substitution line that carries the nonfunctional DTH8 allele) with the Asominori functional DTH8 allele under long-day conditions. DTH8 is expressed in most tissues and its protein is localized to the nucleus exclusively. The quantitative real-time PCR assay revealed that DTH8 could down-regulate the transcriptions of Ehd1 (for Early heading date1) and Hd3a (for Heading date3a; a rice ortholog of FLOWERING LOCUS T) under long-day conditions. Ehd1 and Hd3a can also be down-regulated by the photoperiodic flowering genes Ghd7 and Hd1 (a rice ortholog of CONSTANS). Meanwhile, the transcription of DTH8 has been proved to be independent of Ghd7 and Hd1, and the natural mutation of this gene caused weak photoperiod sensitivity and shorter plant height. Taken together, these data indicate that DTH8 probably plays an important role in the signal network of photoperiodic flowering as a novel suppressor as well as in the regulation of plant height and yield potential.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Mapping Cloning, Molecular Flowers/genetics,growth & development,metabolism Gene Expression Regulation, Plant Genes, Plant Molecular Sequence Data Mutation Oryza/genetics,growth & development,metabolism Photoperiod Plant Proteins/genetics,metabolism Quantitative Trait Loci RNA, Plant/genetics Transcription Factors/genetics,metabolism
Chemicals
Plant Proteins RNA, Plant Transcription Factors
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Wei Xiangjin
State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Provincial Center of Plant Gene Engineering, Nanjing Agricultural University, Nanjing 210095, China.
Xu Junfeng
Guo Hongnian
Jiang Ling
Chen Saihua
Yu Chuanyuan
Zhou Zhenling
Hu Peisong
Zhai Huqu
Wan Jianmin
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
1532-2548
DOI
10.1104/pp.110.156943
Published
2010-08-00
Epub
2010-00-21
Pages
1747-58
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC2923886
Subset
IM
Databases
GENBANK
HM775396, HM775397
External Links
PubMed source DOI: 10.1104/pp.110.156943 PMC Full Text View journal details
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