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PMID: 2072895 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

The early response gene NGFI-C encodes a zinc finger transcriptional activator and is a member of the GCGGGGGCG (GSG) element-binding protein family.

Molecular and cellular biology ·Vol. 11 ·No. 8 ·1991-08-00 ·Pages 3835-41

Crosby SD, Puetz JJ, Simburger KS, Fahrner TJ, Milbrandt J

Abstract

We have cloned NGFI-C, a nerve growth factor-induced early-response gene which encodes a Cys2/His2 zinc finger protein. RNA blot analysis demonstrates that NGFI-C mRNA is induced within minutes of stimulation of PC12 cells by nerve growth factor and is similarly activated in brain after a Metrazol-induced seizure. The cDNA sequence predicts a protein that contains three zinc fingers which show striking homology to the DNA-binding regions of three previously reported zinc finger proteins, NGFI-A, Krox-20, and the Wilms' tumor gene product. NGFI-C binds to the previously described DNA-binding site of these three proteins, which is GCGGGGGCG. Cotransfection experiments revealed that NGFI-C strongly activates transcription from this site in mammalian cells. The isolation of another early-response gene that encodes a member of the G(C/G)G or GSG element-binding family should provide an opportunity to investigate the relative contributions of a family of transcription factors to the cell's response to changes in its environment.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites Cell Line Cloning, Molecular Cycloheximide/pharmacology DNA-Binding Proteins/genetics Genes Molecular Sequence Data Nerve Growth Factors/pharmacology Oligonucleotide Probes Sequence Homology, Nucleic Acid Trans-Activators/genetics Transcription Factors/genetics Zinc Fingers/genetics
Chemicals
DNA-Binding Proteins Nerve Growth Factors Oligonucleotide Probes Trans-Activators Transcription Factors Cycloheximide
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Crosby S D
Department of Pathology, Washington University School of Medicine, St. Louis, Missouri 63110.
Puetz J J
Simburger K S
Fahrner T J
Milbrandt J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.8.3835-3841.1991
Published
1991-08-00
Pages
3835-41
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361165
Subset
IM
Grants
NINDS NIH HHS · IF32NS09005-01 · United States
Databases
GENBANK
D00651, D00652, D00653, D00654, M64825, M64826, M64827, M65008, M92433, S42511
External Links
PubMed source DOI: 10.1128/mcb.11.8.3835-3841.1991 PMC Full Text View journal details
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