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PMID: 20980617 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Zds2p regulates Swe1p-dependent polarized cell growth in Saccharomyces cerevisiae via a novel Cdc55p interaction domain.

Molecular biology of the cell ·Vol. 21 ·No. 24 ·2010-12-00 ·Pages 4373-86

Yasutis K, Vignali M, Ryder M, Tameire F, Dighe SA, Fields S, Kozminski KG

Abstract

Deletion of the paralogs ZDS1 and ZDS2 in the budding yeast Saccharomyces cerevisiae causes a mis-regulation of polarized cell growth. Here we show a function for these genes as regulators of the Swe1p (Wee1p) kinase-dependent G2/M checkpoint. We identified a conserved domain in the C-terminus of Zds2p consisting of amino acids 813-912 (hereafter referred to as ZH4 for Zds homology 4) that is required for regulation of Swe1p-dependent polarized bud growth. ZH4 is shown by protein affinity assays to be necessary and sufficient for interaction with Cdc55p, a regulatory subunit of protein phosphatase 2A (PP2A). We hypothesized that the Zds proteins are in a pathway that negatively regulates the Swe1p-dependent G2/M checkpoint via Cdc55p. Supporting this model, deletion of CDC55 rescues the aberrant bud morphology of a zds1Δzds2Δ strain. We also show that expression of ZDS1 or ZDS2 from a strong galactose-inducible promoter can induce mitosis even when the Swe1p-dependent G2/M checkpoint is activated by mis-organization of the actin cytoskeleton. This negative regulation requires the CDC55 gene. Together these data indicate that the Cdc55p/Zds2p module has a function in the regulation of the Swe1p-dependent G2/M checkpoint.

MeSH Terms
Adaptor Proteins, Signal Transducing/genetics,metabolism Amino Acid Sequence Cell Cycle Proteins/genetics,metabolism Conserved Sequence G2 Phase Gene Deletion Mitosis Mutation, Missense Phosphorylation Protein Interaction Mapping Protein Phosphatase 2/genetics,metabolism Protein-Tyrosine Kinases/genetics,metabolism Recombinant Proteins/genetics,metabolism Saccharomyces cerevisiae/cytology,genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Temperature Time Factors
Chemicals
Adaptor Proteins, Signal Transducing CDC55 protein, S cerevisiae Cell Cycle Proteins Recombinant Proteins Saccharomyces cerevisiae Proteins ZDS1 protein, S cerevisiae ZDS2 protein, S cerevisiae SWE1 protein, S cerevisiae Protein-Tyrosine Kinases Protein Phosphatase 2
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Yasutis Kimberly
Departments of Biology and Cell Biology, University of Virginia, Charlottesville, VA 22904, USA.
Vignali Marissa
Ryder Matthew
Tameire Feven
Dighe Shubha A
Fields Stanley
Kozminski Keith G
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1939-4586
DOI
10.1091/mbc.E10-04-0326
Published
2010-12-00
Epub
2010-00-27
Pages
4373-86
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC3002390
Subset
IM
Grants
NCRR NIH HHS · P41 RR011823 · United States
NIGMS NIH HHS · T32 GM008136 · United States
NIGMS NIH HHS · T32 GM08136 · United States
NCRR NIH HHS · P41 RR11823 · United States
External Links
PubMed source DOI: 10.1091/mbc.E10-04-0326 PMC Full Text View journal details
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