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PMID: 2106668 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Isolation of the chicken middle-molecular weight neurofilament (NF-M) gene and characterization of its promoter.

Nucleic acids research ·Vol. 18 ·No. 3 ·1990-02-11 ·Pages 521-9

Zopf D, Dineva B, Betz H, Gundelfinger ED

Abstract

We have isolated and sequenced genomic DNA clones covering the coding region of the chicken mid-size neurofilament (NF-M) gene and greater than 1 kb of its 5' upstream region. The NF-M gene contains two introns which both are located within the highly conserved C-terminal region of the rod domain. The 5' end of the corresponding mRNA was assigned to a G residue 40 nucleotides upstream of the translation start site and in appropriate distance from a potential TATA box. To functionally analyze the NF-M promoter, constructs carrying 112, 222, and 1026 nucleotides of the 5' upstream region in front of a luciferase reporter gene were tested for their capability to direct luciferase expression after transient transfection into various cell lines. Significant luciferase activity was recorded both in rat phaeochromocytoma (PC12) cells and murine fibroblasts. In PC12 cells, in which neurite outgrowth is induced by nerve growth factor (NGF), expression was stimulated up to 13-fold within 3 days of NGF treatment. This closely resembles expression of the endogenous NF-M gene in response to this hormone.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chickens/genetics Cloning, Molecular DNA/genetics Humans Intermediate Filament Proteins/genetics Mice Molecular Sequence Data Molecular Weight Nerve Growth Factors/pharmacology Neurofilament Proteins Nucleic Acid Hybridization Promoter Regions, Genetic/genetics Rats Regulatory Sequences, Nucleic Acid Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Swine/genetics Transfection
Chemicals
Intermediate Filament Proteins Nerve Growth Factors Neurofilament Proteins DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Zopf D
ZMBH, Universität Heidelberg, FRG.
Dineva B
Betz H
Gundelfinger E D
References (53)
53 references, click to expand
  1. Nerve growth factor activates Thy-1 and neurofilament gene transcription in rat PC12 cells.
    EMBO J. 1986 Dec 20;5(13):3449-53 PMID: 16453742
  2. Structure and evolutionary origin of the gene encoding mouse NF-M, the middle-molecular-mass neurofilament protein.
    Eur J Biochem. 1987 Jul 1;166(1):71-7 PMID: 3036526
  3. Identification and characterization of mRNAs regulated by nerve growth factor in PC12 cells.
    Mol Cell Biol. 1987 Sep;7(9):3156-67 PMID: 3670309
  4. Physiology of nerve growth factor.
    Physiol Rev. 1980 Oct;60(4):1284-335 PMID: 6159658
  5. The structure and organization of the human heavy neurofilament subunit (NF-H) and the gene encoding it.
    EMBO J. 1988 Jul;7(7):1947-55 PMID: 3138108
  6. Site-specific phosphorylation induces disassembly of vimentin filaments in vitro.
    Nature. 1987 Aug 13-19;328(6131):649-52 PMID: 3039376
  7. Neurofilament gene expression: a major determinant of axonal caliber.
    Proc Natl Acad Sci U S A. 1987 May;84(10):3472-6 PMID: 3472217
  8. Signals for the selection of a splice site in pre-mRNA. Computer analysis of splice junction sequences and like sequences.
    J Mol Biol. 1987 May 20;195(2):247-59 PMID: 3656413
  9. Identification of gene products expressed in the developing chick visual system: characterization of a middle-molecular-weight neurofilament cDNA.
    Genes Dev. 1987 Sep;1(7):699-708 PMID: 3123320
  10. Hybrid character of a large neurofilament protein (NF-M): intermediate filament type sequence followed by a long and acidic carboxy-terminal extension.
    EMBO J. 1984 Nov;3(11):2701-6 PMID: 6439558
  11. Transcriptional and post-transcriptional effects of nerve growth factor on expression of the three neurofilament subunits in PC-12 cells.
    J Biol Chem. 1988 Apr 25;263(12):5662-7 PMID: 3128545
  12. Nerve growth factor-induced reduction in epidermal growth factor responsiveness and epidermal growth factor receptors in PC12 cells: an aspect of cell differentiation.
    Biochem Biophys Res Commun. 1979 Jul 12;89(1):175-80 PMID: 314289
  13. Cloning of a cDNA encoding the smallest neurofilament protein from the rat.
    Biochim Biophys Acta. 1985 Aug 21;825(4):398-404 PMID: 3925999
  14. Transient expression of a neurofilament protein by replicating neuroepithelial cells of the embryonic chick brain.
    Dev Biol. 1985 Jan;107(1):107-27 PMID: 3917411
  15. Firefly luciferase gene: structure and expression in mammalian cells.
    Mol Cell Biol. 1987 Feb;7(2):725-37 PMID: 3821727
  16. Structure of the NGFI-A gene and detection of upstream sequences responsible for its transcriptional induction by nerve growth factor.
    Proc Natl Acad Sci U S A. 1989 Jan;86(1):377-81 PMID: 2492104
  17. Arrangement of coding and intervening sequences of chicken lysozyme gene.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6196-200 PMID: 293713
  18. At least two nuclear proteins bind specifically to the Rous sarcoma virus long terminal repeat enhancer.
    Mol Cell Biol. 1987 Feb;7(2):787-98 PMID: 3029568
  19. The complete amino acid sequence of the major mammalian neurofilament protein (NF-L).
    FEBS Lett. 1985 Mar 25;182(2):475-8 PMID: 3920075
  20. Complete amino acid sequence and in vitro expression of rat NF-M, the middle molecular weight neurofilament protein.
    J Neurosci. 1987 Aug;7(8):2590-9 PMID: 2441012
  21. Repetitive satellite-like sequences are present within or upstream from 3 avian protein-coding genes.
    Nucleic Acids Res. 1983 Mar 11;11(5):1227-43 PMID: 6828383
  22. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  23. Structural and functional analysis of a growth-regulated gene, the human calcyclin.
    J Biol Chem. 1987 Jun 15;262(17):8325-32 PMID: 3036810
  24. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
    Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
  25. The human mid-size neurofilament subunit: a repeated protein sequence and the relationship of its gene to the intermediate filament gene family.
    EMBO J. 1987 Jun;6(6):1617-26 PMID: 3608989
  26. Location and sequence characterization of the major phosphorylation sites of the high molecular mass neurofilament proteins M and H.
    FEBS Lett. 1987 Sep 14;221(2):403-7 PMID: 3114005
  27. The structure of the largest murine neurofilament protein (NF-H) as revealed by cDNA and genomic sequences.
    Brain Res. 1988 Nov;464(3):217-31 PMID: 3145094
  28. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
    Proc Natl Acad Sci U S A. 1976 Jul;73(7):2424-8 PMID: 1065897
  29. High-efficiency transformation of mammalian cells by plasmid DNA.
    Mol Cell Biol. 1987 Aug;7(8):2745-52 PMID: 3670292
  30. Developmental accumulation and heterogeneity of myelin basic protein transcripts in the chick visual system.
    Glia. 1989;2(4):241-9 PMID: 2475444
  31. Rat prostatic binding protein: the complete sequence of the C2 gene and its flanking regions.
    Nucleic Acids Res. 1987 Feb 25;15(4):1627-41 PMID: 2881277
  32. A structural basis for S1 nuclease sensitivity of double-stranded DNA.
    Cell. 1985 Aug;42(1):271-80 PMID: 2990727
  33. Anomalous placement of introns in a member of the intermediate filament multigene family: an evolutionary conundrum.
    Mol Cell Biol. 1986 May;6(5):1529-34 PMID: 3785173
  34. Two adjacent promoter elements mediate nerve growth factor activation of the c-fos gene and bind distinct nuclear complexes.
    Proc Natl Acad Sci U S A. 1988 Dec;85(24):9474-8 PMID: 2849107
  35. Expression and assembly of a human neurofilament protein in transgenic mice provide a novel neuronal marking system.
    Genes Dev. 1987 Dec;1(10):1085-95 PMID: 2828164
  36. Structure of the gene encoding peripherin, an NGF-regulated neuronal-specific type III intermediate filament protein.
    Neuron. 1989 Jan;2(1):1043-53 PMID: 2624740
  37. Regulation of inducible and tissue-specific gene expression.
    Science. 1987 Jun 5;236(4806):1237-45 PMID: 3296191
  38. Initial expression of neurofilaments and vimentin in the central and peripheral nervous system of the mouse embryo in vivo.
    J Neurosci. 1984 Aug;4(8):2080-94 PMID: 6432971
  39. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  40. Levels of nerve growth factor and its mRNA in the central nervous system of the rat correlate with cholinergic innervation.
    EMBO J. 1985 Jun;4(6):1389-93 PMID: 2411537
  41. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  42. Chicken hematopoietic cells transformed by seven strains of defective avian leukemia viruses display three distinct phenotypes of differentiation.
    Cell. 1979 Oct;18(2):375-90 PMID: 227607
  43. Cloning of a cDNA encoding the rat high molecular weight neurofilament peptide (NF-H): developmental and tissue expression in the rat, and mapping of its human homologue to chromosomes 1 and 22.
    Proc Natl Acad Sci U S A. 1989 Apr;86(7):2463-7 PMID: 2928342
  44. The structure of a human neurofilament gene (NF-L): a unique exon-intron organization in the intermediate filament gene family.
    Biochim Biophys Acta. 1987 Jun 6;909(1):10-20 PMID: 3034332
  45. Nerve growth factor and epidermal growth factor induce rapid transient changes in proto-oncogene transcription in PC12 cells.
    J Biol Chem. 1985 Nov 15;260(26):14101-10 PMID: 3877054
  46. The large neurofilament subunit (NF-H) of the rat: cDNA cloning and in situ detection.
    Biochem Biophys Res Commun. 1988 Aug 15;154(3):1099-106 PMID: 2457365
  47. Phosphorylation of desmin in vitro inhibits formation of intermediate filaments; identification of three kinase A sites in the aminoterminal head domain.
    EMBO J. 1988 Jan;7(1):15-20 PMID: 3359992
  48. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  49. Expression of NF-L and NF-M in fibroblasts reveals coassembly of neurofilament and vimentin subunits.
    J Cell Biol. 1989 Feb;108(2):579-93 PMID: 2493000
  50. Protein-chemical characterization of NF-H, the largest mammalian neurofilament component; intermediate filament-type sequences followed by a unique carboxy-terminal extension.
    EMBO J. 1985 Jan;4(1):57-63 PMID: 16453600
  51. Nerve growth factor.
    Physiol Rev. 1968 Jul;48(3):534-69 PMID: 4875350
  52. Cloning and developmental expression of the murine neurofilament gene family.
    Brain Res. 1986 Dec;387(3):243-50 PMID: 3103856
  53. CpG-rich islands and the function of DNA methylation.
    Nature. 1986 May 15-21;321(6067):209-13 PMID: 2423876
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.3.521
Published
1990-02-11
Pages
521-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333457
Subset
IM
Databases
GENBANK
X17102
External Links
PubMed source DOI: 10.1093/nar/18.3.521 PMC Full Text View journal details
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