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PMID: 2108259 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effects of a highly basic region of human immunodeficiency virus Tat protein on nucleolar localization.

Journal of virology ·Vol. 64 ·No. 4 ·1990-04-00 ·Pages 1803-7

Siomi H, Shida H, Maki M, Hatanaka M

Abstract

Human immunodeficiency virus type 1 encodes a positive trans-activator protein, Tat, which is located predominantly in the cell nucleolus. To study the role of the basic region of Tat in nucleolar localization, we constructed fusion genes encoding serially deleted segments of Tat joined to the amino-terminal end of the Escherichia coli beta-galactosidase molecule. We show that the basic region of Tat was sufficient for nuclear localization but not for nucleolar localization. Addition of three amino acids (59, 60, and 61) of the Tat sequence at the C-terminal end of the basic region was necessary for the chimeric beta-galactosidase to localize in the nucleus as well as in the nucleolus. We demonstrate that a short amino acid sequence (G-48 RKKRRQRRRA HQ N-61), when fused to the amino terminus of beta-galactosidase, can act as a nucleolar localization signal.

MeSH Terms
Amino Acid Sequence Animals Cell Nucleolus/metabolism Cell Nucleus/metabolism Fluorescent Antibody Technique Gene Products, tat/metabolism HIV-1 Lac Operon Molecular Sequence Data Recombinant Fusion Proteins/metabolism Trans-Activators/metabolism beta-Galactosidase/genetics tat Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, tat Recombinant Fusion Proteins Trans-Activators tat Gene Products, Human Immunodeficiency Virus beta-Galactosidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Siomi H
Institute for Virus Research, Kyoto University, Japan.
Shida H
Maki M
Hatanaka M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.4.1803-1807.1990
Published
1990-04-00
Pages
1803-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249318
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.64.4.1803-1807.1990 PMC Full Text View journal details
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