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PMID: 2108945 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of epidermal growth factor, transforming growth factor-alpha and their receptor genes in human gastric carcinomas; implication for autocrine growth.

Japanese journal of cancer research : Gann ·Vol. 81 ·No. 1 ·1990-01-00 ·Pages 43-51

Yoshida K, Kyo E, Tsujino T, Sano T, Niimoto M, Tahara E

Abstract

The expressions of mRNA for epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha) and EGF receptor (EGFR) genes were examined in 7 human gastric carcinoma cell lines and 15 gastric carcinoma tissues and the corresponding normal mucosas. All of the gastric carcinoma cell lines expressed mRNA for EGFR and TGF-alpha genes. TMK-1 and MKN-28 cells also expressed EGF mRNA. Production of EGF, TGF-alpha and EGFR protein by gastric carcinoma cell lines was also confirmed by EGF and TGF-alpha specific monoclonal antibody binding. As for surgical specimens, EGFR and TGF-alpha mRNA were detected at high levels in all the tumor tissues. Interestingly, EGF mRNA was detected in 5 (33.3%) of the 15 gastric carcinomas but it was not detected in normal tissues. Moreover, anti-EGF and anti-TGF-alpha monoclonal antibodies inhibited the spontaneous 3H-TdR uptake by gastric carcinoma cells. These results suggest that EGF and/or TGF-alpha produced by tumor cells act as autocrine growth factors for gastric carcinomas.

MeSH Terms
Antibodies, Monoclonal/pharmacology DNA, Neoplasm/biosynthesis Epidermal Growth Factor/genetics,immunology ErbB Receptors/genetics Gene Expression Humans Immunoassay RNA, Messenger/genetics,metabolism Stomach Neoplasms/genetics,pathology Transforming Growth Factors/genetics,immunology Tumor Cells, Cultured
Chemicals
Antibodies, Monoclonal DNA, Neoplasm RNA, Messenger Epidermal Growth Factor Transforming Growth Factors ErbB Receptors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Yoshida K
First Department of Pathology, Hiroshima University School of Medicine.
Kyo E
Tsujino T
Sano T
Niimoto M
Tahara E
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46 references, click to expand
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Article Info
Journal
Japanese journal of cancer research : Gann
Abbr.
Jpn J Cancer Res
ISSN
0910-5050
DOI
10.1111/j.1349-7006.1990.tb02505.x
Published
1990-01-00
Pages
43-51
Language
English
Region
Japan
NLM ID
8509412
PMCID
PMC5917953
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1349-7006.1990.tb02505.x PMC Full Text
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