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PMID: 2117575 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Oral immunization with recombinant Streptococcus lactis carrying the Streptococcus mutans surface protein antigen gene.

Infection and immunity ·Vol. 58 ·No. 9 ·1990-09-00 ·Pages 2929-34

Iwaki M, Okahashi N, Takahashi I, Kanamoto T, Sugita-Konishi Y, Aibara K, Koga T

Abstract

A recombinant Streptococcus lactis strain which carries the structural gene for a surface protein antigen (PAc) of 190,000 daltons from Streptococcus mutans serotype c was constructed for development of an oral vaccine against dental caries. The gene from S. mutans MT8148 joined to shuttle vector pSA3 was successfully transformed into S. lactis IL1403. A small amount of PAc was detected in the cell homogenate and cytoplasmic fraction of the recombinant S. lactis, but not in the culture supernatant of the recombinant, by Western immunoblotting and dot immunoblotting. The level of PAc-specific mRNA in the recombinant strain was lower than that in S. mutans MT8148. However, significant salivary immunoglobulin A and serum immunoglobulin G responses to PAc were induced in mice immunized orally with the recombinant S. lactis.

MeSH Terms
Animals Antigens, Bacterial/genetics Bacterial Vaccines/genetics Blotting, Southern Cloning, Molecular Dental Caries/immunology Escherichia coli/genetics Gene Expression Genes, Bacterial Immunoglobulins/immunology Lactococcus lactis/genetics,immunology Male Mice Mice, Inbred BALB C Plasmids RNA, Messenger/metabolism Recombination, Genetic Saliva/immunology Streptococcus mutans/genetics,immunology Transcription, Genetic Vaccines/genetics Vaccines, Synthetic/genetics
Chemicals
Antigens, Bacterial Bacterial Vaccines Immunoglobulins RNA, Messenger Vaccines Vaccines, Synthetic
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Iwaki M
Department of Biomedical Research on Foods, National Institute of Health, Tokyo, Japan.
Okahashi N
Takahashi I
Kanamoto T
Sugita-Konishi Y
Aibara K
Koga T
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.58.9.2929-2934.1990
Published
1990-09-00
Pages
2929-34
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313589
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.58.9.2929-2934.1990 PMC Full Text View journal details
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