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PMID: 2118103 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The molecular characterization of PRP6 and PRP9 yeast genes reveals a new cysteine/histidine motif common to several splicing factors.

The EMBO journal ·Vol. 9 ·No. 9 ·1990-09-00 ·Pages 2775-81

Legrain P, Choulika A

Abstract

prp6 and prp9 thermosensitive (ts) mutants are affected in pre-mRNA splicing and transport from the nucleus to the cytoplasm. PRP6 and PRP9 wild-type alleles have been sequenced. DNA sequence analysis reveals homologies in the 5' and 3' non-coding regions, suggesting a common regulation of gene expression. PRP6 and PRP9 genes encode a 899 amino acid and a 530 amino acid protein, respectively. The PRP6 protein has repeated motifs that evoke helix-loop-helix structures. Both PRP6 and PRP9 proteins have cysteine/histidine motifs loosely related to those found in zinc finger proteins. The substitution of some, but not all, of these residues by directed mutagenesis has a critical effect on the protein function. Homology searches reveal that two other proteins known to be involved in the nuclear splicing pathway--the yeast PRP11 and the human U1C proteins--contain similar sequences. The five cysteine/histidine motifs found in these four proteins display amino acid similarities in addition to the cysteine and histidine residues, indicating that they participate in biological structures or functions related to the splicing process. In addition, PRP6 and PRP9 exhibit leucine repeat motifs which may be implicated in protein interactions. The prp6 and prp9 ts mutations have been mapped and sequenced.

MeSH Terms
Alleles Amino Acid Sequence Base Sequence Cysteine DNA, Fungal/genetics DNA-Binding Proteins/genetics Fungal Proteins/genetics Genes, Fungal Humans Metalloproteins/genetics Molecular Sequence Data Mutation Plasmids Protein Conformation RNA Splicing RNA Splicing Factors RNA-Binding Proteins Restriction Mapping Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Homology, Nucleic Acid Zinc/metabolism
Chemicals
DNA, Fungal DNA-Binding Proteins Fungal Proteins Metalloproteins PRP9 protein, S cerevisiae RNA Splicing Factors RNA-Binding Proteins Saccharomyces cerevisiae Proteins Zinc Cysteine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Legrain P
Department of Molecular Biology, Institut Pasteur, Paris, France.
Choulika A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-09-00
Pages
2775-81
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551986
Subset
IM
Databases
GENBANK
X53465, X53466
External Links
PubMed source PMC Full Text View journal details
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