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PMID: 2123027 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of three different repeated DNA elements present in the P1 operon of Mycoplasma pneumoniae: size, number and distribution on the genome.

Nucleic acids research ·Vol. 18 ·No. 21 ·1990-11-11 ·Pages 6311-7

Ruland K, Wenzel R, Herrmann R

Abstract

Mycoplasma pneumoniae, a bacterium pathogenic for humans, has a relatively small genome size of 840 kbp. Even though, several repeated DNA elements have been identified in the genome of this prokaryote, particularly within the P1 gene which codes for a major adhesin protein of M. pneumoniae. These elements were characterized in detail with respect to size, number and distribution on the genome, represented by an ordered cloned library covering the complete chromosome. Three different repetitive elements were detected in and around the P1 gene designated as RepMP2/3, RepMP4 and RepMP5. The length of these elements varies between 1.1-1.5 kbp (RepMP4), 1.8 kbp (RepMP2/3) and 1.9-2.2 kpb (RepMP5). They occur at least 8 to 10 times on the chromosome. Possible functions are discussed and a uniform nomenclature for these repeats is proposed.

MeSH Terms
Blotting, Southern Cloning, Molecular/methods Cosmids DNA Probes DNA, Bacterial/genetics,isolation & purification Genes, Bacterial Genomic Library Mycoplasma pneumoniae/genetics Operon Repetitive Sequences, Nucleic Acid Restriction Mapping
Chemicals
DNA Probes DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ruland K
Department of Microbiology, University of Heidelberg, FRG.
Wenzel R
Herrmann R
References (24)
24 references, click to expand
  1. The completeness of molecular biology.
    Isr J Med Sci. 1984 Sep;20(9):750-3 PMID: 6511349
  2. Repetitive extragenic palindromic sequences: a major component of the bacterial genome.
    Cell. 1984 Jul;37(3):1015-26 PMID: 6378385
  3. Transpositional recombination in prokaryotes.
    Annu Rev Biochem. 1985;54:863-96 PMID: 2992361
  4. What are mycoplasmas: the relationship of tempo and mode in bacterial evolution.
    J Mol Evol. 1984-1985;21(4):305-16 PMID: 6085735
  5. The repertoire of silent pilus genes in Neisseria gonorrhoeae: evidence for gene conversion.
    Cell. 1986 Jan 17;44(1):107-15 PMID: 2866848
  6. Molecular biology and genetics of mycoplasmas (Mollicutes).
    Microbiol Rev. 1985 Dec;49(4):419-55 PMID: 3912655
  7. The principal protein antigens of isolates of Mycoplasma pneumoniae as measured by levels of immunoglobulin G in human serum are stable in strains collected over a 10-year period.
    Infect Immun. 1987 Aug;55(8):1830-6 PMID: 3112011
  8. Cloning and sequence analysis of cytadhesin P1 gene from Mycoplasma pneumoniae.
    Infect Immun. 1987 Dec;55(12):3023-9 PMID: 3119495
  9. Pulsed-field electrophoresis indicates larger-than-expected sizes for mycoplasma genomes.
    Nucleic Acids Res. 1988 Jul 11;16(13):6015-25 PMID: 2840639
  10. Nucleotide sequence of the P1 attachment-protein gene of Mycoplasma pneumoniae.
    Gene. 1988 Apr 29;64(2):217-29 PMID: 2841195
  11. Parameters of Mycoplasma pneumoniae infection in Syrian hamsters.
    Infect Immun. 1988 Sep;56(9):2443-9 PMID: 3137169
  12. Physical mapping of the Mycoplasma pneumoniae genome.
    Nucleic Acids Res. 1988 Sep 12;16(17):8323-36 PMID: 2843814
  13. Repetitive DNA sequences in Mycoplasma pneumoniae.
    Nucleic Acids Res. 1988 Sep 12;16(17):8337-50 PMID: 3138660
  14. Regions of Mycoplasma pneumoniae cytadhesin P1 structural gene exist as multiple copies.
    Infect Immun. 1988 Dec;56(12):3157-61 PMID: 3141282
  15. Reiterated DNA sequences defining genomic diversity within the species Mycoplasma hyorhinis.
    Mol Microbiol. 1988 Sep;2(5):665-72 PMID: 2460718
  16. Anomalous values of Mycoplasma genomes sizes determined by pulse-field gel electrophoresis.
    Nucleic Acids Res. 1989 Feb 11;17(3):1268 PMID: 2922272
  17. Analysis of the nucleotide sequence of the P1 operon of Mycoplasma pneumoniae.
    Gene. 1988 Dec 15;73(1):175-83 PMID: 2468577
  18. Cloning of the complete Mycoplasma pneumoniae genome.
    Nucleic Acids Res. 1989 Sep 12;17(17):7029-43 PMID: 2506532
  19. Prevalence of novel repeat sequences in and around the P1 operon in the genome of Mycoplasma pneumoniae.
    Gene. 1990 Mar 1;87(1):91-6 PMID: 2110102
  20. Tissue cultures and mycoplasmas.
    Tex Rep Biol Med. 1965 Jun;23:Suppl 1:285+ PMID: 5833547
  21. Recombinational switch for gene expression.
    Science. 1977 Apr 8;196(4286):170-2 PMID: 322276
  22. Mycoplasma pneumoniae infection: role of a surface protein in the attachment organelle.
    Science. 1982 Apr 16;216(4543):313-5 PMID: 6801766
  23. Transposable elements in prokaryotes.
    Annu Rev Genet. 1981;15:341-404 PMID: 6279020
  24. Antigenic variation is associated with DNA rearrangements in a relapsing fever Borrelia.
    Cell. 1985 Jun;41(2):403-9 PMID: 2580643
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.21.6311
Published
1990-11-11
Pages
6311-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC332497
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/18.21.6311 PMC Full Text View journal details
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