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PMID: 21245079 Published · ppublish English Comparative Study Journal Article Review

Base-calling for next-generation sequencing platforms.

Briefings in bioinformatics ·Vol. 12 ·No. 5 ·2011-09-00 ·Pages 489-97

Ledergerber C, Dessimoz C

Abstract

Next-generation sequencing platforms are dramatically reducing the cost of DNA sequencing. With these technologies, bases are inferred from light intensity signals, a process commonly referred to as base-calling. Thus, understanding and improving the quality of sequence data generated using these approaches are of high interest. Recently, a number of papers have characterized the biases associated with base-calling and proposed methodological improvements. In this review, we summarize recent development of base-calling approaches for the Illumina and Roche 454 sequencing platforms.

MeSH Terms
Animals Base Sequence Humans Sequence Analysis, DNA/methods
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ledergerber Christian
ETH Zurich, Switzerland.
Dessimoz Christophe
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26 references, click to expand
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Article Info
Journal
Briefings in bioinformatics
Abbr.
Brief Bioinform
ISSN
1477-4054
DOI
10.1093/bib/bbq077
Published
2011-09-00
Epub
2011-00-18
Pages
489-97
Language
English
Region
England
NLM ID
100912837
PMCID
PMC3178052
Subset
IM
External Links
PubMed source DOI: 10.1093/bib/bbq077 PMC Full Text View journal details
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