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PMID: 2125210 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cloning, sequencing and analysis of the structural gene and regulatory region of the Pseudomonas aeruginosa chromosomal ampC beta-lactamase.

The Biochemical journal ·Vol. 272 ·No. 3 ·1990-12-15 ·Pages 627-31

Lodge JM, Minchin SD, Piddock LJ, Busby SJ

Abstract

The chromosomal gene from Pseudomonas aeruginosa encoding beta-lactamase has been cloned, and the sequence determined and compared with corresponding sequences of beta-lactamases from members of the enterobacteriaceae. Upstream of the beta-lactamase gene is an open reading frame which we postulate encodes a regulatory protein, AmpR. We identified a helix-turn-helix region in AmpR and a putative AmpR-binding site.

Related Genes
ampC
MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Genes, Bacterial Genes, Regulator Molecular Sequence Data Open Reading Frames Pseudomonas aeruginosa/enzymology,genetics Restriction Mapping Sequence Homology, Nucleic Acid beta-Lactamases/genetics
Chemicals
beta-Lactamases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lodge J M
School of Biochemistry, University of Birmingham, U.K.
Minchin S D
Piddock L J
Busby S J
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26 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2720627
Published
1990-12-15
Pages
627-31
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1149754
Subset
IM
Databases
GENBANK
X54719
External Links
PubMed source DOI: 10.1042/bj2720627 PMC Full Text View journal details
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