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PMID: 212592 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Viral gene expression in murine sarcoma virus(murine leukemia virus)-infected cells.

Journal of virology ·Vol. 27 ·No. 3 ·1978-09-00 ·Pages 768-75

Dina D, Penhoet EE

Abstract

NIH 3T3 cells infected with Moloney murine sarcoma virus (murine leukemia virus) produce virions which contain about 99% murine sarcoma virus RNA and 1% murine leukemia virus RNA. This report describes experiments which measured intracellular concentrations of proviral DNA and RNA transcripts for each of the viruses. We found that three to four copies of proviral DNA from each virus were integrated into the cellular DNA. Measurements of RNA specific for each of the genomes by hybridization to specific cDNA reagents revealed a 10- to 15-fold difference in concentration in both nuclear and polysomal RNA fractions, with murine sarcoma virus RNA predominating in both cases. Unless there are major differences in stability between the two viral RNAs, our results suggest that transcriptional control is responsible for much of the difference in final levels of virus synthesis.

MeSH Terms
Cell Line Cell Transformation, Neoplastic Cell Transformation, Viral DNA, Viral/biosynthesis Gammaretrovirus/genetics Genes, Viral Moloney murine leukemia virus/genetics,growth & development,metabolism RNA, Viral/biosynthesis,genetics Sarcoma Viruses, Murine/genetics,growth & development,metabolism Transcription, Genetic Virus Replication
Chemicals
DNA, Viral RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dina D
Penhoet E E
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35 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.27.3.768-775.1978
Published
1978-09-00
Pages
768-75
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC525864
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.27.3.768-775.1978 PMC Full Text View journal details
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