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PMID: 21263038 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genetic control of a transition from black to straw-white seed hull in rice domestication.

Plant physiology ·Vol. 155 ·No. 3 ·2011-03-00 ·Pages 1301-11

Zhu BF, Si L, Wang Z, Zhou Y, Zhu J, Shangguan Y, Lu D, Fan D, Li C, Lin H, Qian Q, Sang T, Zhou B, Minobe Y, Han B

Abstract

The genetic mechanism involved in a transition from the black-colored seed hull of the ancestral wild rice (Oryza rufipogon and Oryza nivara) to the straw-white seed hull of cultivated rice (Oryza sativa) during grain ripening remains unknown. We report that the black hull of O. rufipogon was controlled by the Black hull4 (Bh4) gene, which was fine-mapped to an 8.8-kb region on rice chromosome 4 using a cross between O. rufipogon W1943 (black hull) and O. sativa indica cv Guangluai 4 (straw-white hull). Bh4 encodes an amino acid transporter. A 22-bp deletion within exon 3 of the bh4 variant disrupted the Bh4 function, leading to the straw-white hull in cultivated rice. Transgenic study indicated that Bh4 could restore the black pigment on hulls in cv Guangluai 4 and Kasalath. Bh4 sequence alignment of all taxa with the outgroup Oryza barthii showed that the wild rice maintained comparable levels of nucleotide diversity that were about 70 times higher than those in the cultivated rice. The results from the maximum likelihood Hudson-Kreitman-Aguade test suggested that the significant reduction in nucleotide diversity in rice cultivars could be caused by artificial selection. We propose that the straw-white hull was selected as an important visual phenotype of nonshattered grains during rice domestication.

MeSH Terms
Agriculture Amino Acid Transport Systems Cloning, Molecular Crops, Agricultural/genetics Gene Expression Regulation, Plant Genes, Plant/genetics Geography Inbreeding Molecular Sequence Data Mutation/genetics Nucleotides/genetics Organ Specificity/genetics Oryza/genetics Phenotype Phylogeny Pigmentation/genetics Plant Proteins/genetics,metabolism Plants, Genetically Modified RNA, Messenger/genetics,metabolism Seeds/anatomy & histology,genetics Sequence Homology, Nucleic Acid
Chemicals
Amino Acid Transport Systems Nucleotides Plant Proteins RNA, Messenger
Authors & Affiliations
15 authors, click to expand affiliations / ORCID
Zhu Bo-Feng
National Center for Gene Research and Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China.
Si Lizhen
Wang Zixuan
Zhou Yan
Zhu Jinjie
Shangguan Yingying
Lu Danfeng
Fan Danlin
Li Canyang
Lin Hongxuan
Qian Qian
Sang Tao
Zhou Bo
Minobe Yuzo
Han Bin
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
1532-2548
DOI
10.1104/pp.110.168500
Published
2011-03-00
Epub
2011-00-24
Pages
1301-11
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC3046587
Subset
IM
Databases
GENBANK
FQ377518, FQ377519, FQ377520, FQ377521, FQ377522, FQ377523, FQ377524, FQ377525, FQ377526, FQ377527, FQ377528, FQ377529, FQ377530, FQ377531, FQ377532, FQ377533, FQ377534, FQ377535, FQ377536, FQ377537, FQ377538, FQ377539, FQ377540, FQ377541, FQ377542, FQ377543, FQ377544, FQ377545, FQ377546, FQ377547, FQ377548, FQ377549, FQ377550, FQ377551, FQ377552, FQ377553, FQ377554, FQ377555, FQ377556, FQ377557, FQ377558, FQ377559, FQ377560, FQ377561, FQ377562, FQ377563, FQ377564, FQ377565, FQ377566, FQ377579, FQ377580, FQ377581, FQ377582, FQ377583, FQ377585
External Links
PubMed source DOI: 10.1104/pp.110.168500 PMC Full Text View journal details
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