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PMID: 2137554 Published · ppublish English Journal Article

Nuclear transport of proteins translated in vitro from SP6 plasmid-generated mRNAs.

Molecular and cellular biology ·Vol. 10 ·No. 3 ·1990-03-00 ·Pages 1287-92

Parnaik VK, Kennady PK

Abstract

A sensitive and versatile assay is described for the nuclear transport of 35S-labeled proteins obtained by the in vitro translation of SP6 plasmid-generated mRNAs. A specific nuclear accumulation of greater than 20-fold is observed for the transformation-related nuclear proteins, p53 and E1b, and the nuclear enzyme, thymidine kinase, whereas transport of the nonnuclear proteins, dihydrofolate reductase and simian virus 40 small t antigen, is negligible within 30 min.

MeSH Terms
Adenosine Triphosphate/metabolism Adenovirus Early Proteins Biological Transport Cell Nucleus/metabolism Cell-Free System Cytoplasm/physiology DNA, Recombinant Fluorescent Antibody Technique In Vitro Techniques Nuclear Proteins/metabolism Oncogene Proteins/metabolism Oncogene Proteins, Viral/metabolism Phosphoproteins/metabolism Plasmids Protein Biosynthesis Thymidine Kinase/metabolism Tumor Suppressor Protein p53
Chemicals
Adenovirus Early Proteins DNA, Recombinant Nuclear Proteins Oncogene Proteins Oncogene Proteins, Viral Phosphoproteins Tumor Suppressor Protein p53 Adenosine Triphosphate Thymidine Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Parnaik V K
Centre for Cellular and Molecular Biology, Hyderabad, India.
Kennady P K
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.3.1287-1292.1990
Published
1990-03-00
Pages
1287-92
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361022
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.10.3.1287-1292.1990 PMC Full Text View journal details
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