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PMID: 21376234 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation.

Cell ·Vol. 144 ·No. 5 ·2011-03-04 ·Pages 719-31

Pan J, Sasaki M, Kniewel R, Murakami H, Blitzblau HG, Tischfield SE, Zhu X, Neale MJ, Jasin M, Socci ND, Hochwagen A, Keeney S

Abstract

The nonrandom distribution of meiotic recombination influences patterns of inheritance and genome evolution, but chromosomal features governing this distribution are poorly understood. Formation of the DNA double-strand breaks (DSBs) that initiate recombination results in the accumulation of Spo11 protein covalently bound to small DNA fragments. By sequencing these fragments, we uncover a genome-wide DSB map of unprecedented resolution and sensitivity. We use this map to explore how DSB distribution is influenced by large-scale chromosome structures, chromatin, transcription factors, and local sequence composition. Our analysis offers mechanistic insight into DSB formation and early processing steps, supporting the view that the recombination terrain is molded by combinatorial and hierarchical interaction of factors that work on widely different size scales. This map illuminates the occurrence of DSBs in repetitive DNA elements, repair of which can lead to chromosomal rearrangements. We also discuss implications for evolutionary dynamics of recombination hot spots.

MeSH Terms
DNA Breaks, Double-Stranded Endodeoxyribonucleases/metabolism Genome, Fungal Genome-Wide Association Study Recombination, Genetic Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins/metabolism
Chemicals
Saccharomyces cerevisiae Proteins Endodeoxyribonucleases Spo11 protein, S cerevisiae
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Pan Jing
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.
Sasaki Mariko
Kniewel Ryan
Murakami Hajime
Blitzblau Hannah G
Tischfield Sam E
Zhu Xuan
Neale Matthew J
Jasin Maria
Socci Nicholas D
Hochwagen Andreas
Keeney Scott
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
1097-4172
DOI
10.1016/j.cell.2011.02.009
Published
2011-03-04
Pages
719-31
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC3063416
Subset
IM
Grants
NICHD NIH HHS · R01 HD053855-02 · United States
Howard Hughes Medical Institute · United States
NIGMS NIH HHS · R01 GM058673-08 · United States
NIGMS NIH HHS · T32 GM008539 · United States
NIGMS NIH HHS · GM58673 · United States
NICHD NIH HHS · HD53855 · United States
NIGMS NIH HHS · R01 GM058673 · United States
Medical Research Council · G0800005 · United Kingdom
NICHD NIH HHS · R01 HD053855 · United States
External Links
PubMed source DOI: 10.1016/j.cell.2011.02.009 PMC Full Text View journal details
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