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PMID: 2139141 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The adenovirus E4 17-kilodalton protein complexes with the cellular transcription factor E2F, altering its DNA-binding properties and stimulating E1A-independent accumulation of E2 mRNA.

Journal of virology ·Vol. 64 ·No. 5 ·1990-05-00 ·Pages 2345-59

Marton MJ, Baim SB, Ornelles DA, Shenk T

Abstract

E2F is a cellular DNA-binding factor. Its binding activity is changed within adenovirus-infected cells so that it binds cooperatively to pairs of properly spaced and oriented E2F recognition sites. In the work described in this report, the conversion to cooperative binding was shown to require the adenovirus E4 17-kilodalton (kDa) polypeptide. Mutant viruses carrying alterations within the E4 17-kDa coding region failed to generate the infection-specific, cooperatively binding form of E2F. It was possible to alter E2F from uninfected cells so that it bound cooperatively by incubation with a partially purified fraction obtained from infected cells. The E4 17-kDa protein copurified with this activity and was also found to be present in a complex containing E2F. Consistent with its ability to alter the binding of E2F to its recognition sites within the E2 promoter, the E4 17-kDa polypeptide contributed to maximal expression of E2 mRNAs in some cell types. Its ability to enhance E2 transcription did not require expression of the E1A transactivator protein. These results are consistent with a model which proposes that the E4 17-kDa polypeptide binds to the cellular E2F factor, altering its binding behavior and thereby enhancing its ability to stimulate transcription.

MeSH Terms
Adenovirus Early Proteins Adenoviruses, Human/genetics Amino Acid Sequence Animals Base Sequence Cell Line Chromosome Mapping DNA, Viral/genetics,isolation & purification DNA-Binding Proteins/metabolism Gene Expression Regulation, Viral Genes, Viral HeLa Cells/metabolism Humans Molecular Sequence Data Mutation Oligonucleotide Probes Oncogene Proteins, Viral/genetics,metabolism RNA, Messenger/genetics Transcription Factors/metabolism Transcription, Genetic Viral Structural Proteins/genetics
Chemicals
Adenovirus Early Proteins DNA, Viral DNA-Binding Proteins Oligonucleotide Probes Oncogene Proteins, Viral RNA, Messenger Transcription Factors Viral Structural Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Marton M J
Department of Biology, Howard Hughes Medical Institute, Princeton University, New Jersey 08544-1014.
Baim S B
Ornelles D A
Shenk T
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.5.2345-2359.1990
Published
1990-05-00
Pages
2345-59
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249396
Subset
IM
Grants
NCI NIH HHS · CA 38965 · United States
NIGMS NIH HHS · GM 07312 · United States
External Links
PubMed source DOI: 10.1128/JVI.64.5.2345-2359.1990 PMC Full Text View journal details
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