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PMID: 2144608 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Functional analysis of the murine T-cell receptor beta enhancer and characteristics of its DNA-binding proteins.

Molecular and cellular biology ·Vol. 10 ·No. 10 ·1990-10-00 ·Pages 5027-35

Takeda J, Cheng A, Mauxion F, Nelson CA, Newberry RD, Sha WC, Sen R, Loh DY

Abstract

The minimal T-cell receptor (TCR) beta-chain (TCR beta) enhancer has been identified by transfection into lymphoid cells. The minimal enhancer was active in T cells and in some B-lineage cells. When a larger fragment containing the minimal enhancer was used, its activity was apparent only in T cells. Studies with phytohemagglutinin and 4 beta-phorbol-12,13-dibutyrate revealed that the enhancer activity was increased by these agents. By a combination of DNase I footprinting, gel mobility shift assay, and methylation interference analysis, seven different motifs were identified within the minimal enhancer. Furthermore, competition experiments showed that some of these elements bound identical or similar factors that are known to bind to the TCR V beta promoter decamer or to the immunoglobulin enhancer kappa E2 or muEBP-E motif. These shared motifs may be important in the differential gene activity among the different lymphoid subsets.

MeSH Terms
Animals Base Sequence Binding Sites Cloning, Molecular DNA Mutational Analysis DNA-Binding Proteins/physiology Enhancer Elements, Genetic Gene Expression Regulation Lymphocytes/physiology Mice Molecular Sequence Data Phorbol 12,13-Dibutyrate/pharmacology Phytohemagglutinins/pharmacology Promoter Regions, Genetic Receptors, Antigen, T-Cell/genetics Receptors, Antigen, T-Cell, alpha-beta Restriction Mapping Structure-Activity Relationship
Chemicals
DNA-Binding Proteins Phytohemagglutinins Receptors, Antigen, T-Cell Receptors, Antigen, T-Cell, alpha-beta Phorbol 12,13-Dibutyrate
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Takeda J
Howard Hughes Medical Institute, Washington University School of Medicine, St. Louis, Missouri 63110.
Cheng A
Mauxion F
Nelson C A
Newberry R D
Sha W C
Sen R
Loh D Y
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.10.5027-5035.1990
Published
1990-10-00
Pages
5027-35
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361162
Subset
IM
Grants
NIAID NIH HHS · AI15353 · United States
NIGMS NIH HHS · GM38925 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.10.5027-5035.1990 PMC Full Text View journal details
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