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PMID: 2148564 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cholesterol controls the clustering of the glycophospholipid-anchored membrane receptor for 5-methyltetrahydrofolate.

The Journal of cell biology ·Vol. 111 ·No. 6 Pt 2 ·1990-12-00 ·Pages 2931-8

Rothberg KG, Ying YS, Kamen BA, Anderson RG

Abstract

The folate receptor is a glycosyl-phosphatidylinositol (GPI)-anchored membrane protein that mediates the delivery of 5-methyltetrahydrofolate to the cytoplasm of MA104 cells. Ordinarily the receptor is sequestered into numerous discrete clusters that are associated with an uncoated pit membrane specialization called a caveola. By using two different methodological approaches, we found that the maintenance of both receptor clusters and caveolae depends upon the presence of cholesterol in the membrane. These results suggest that cholesterol plays a critical role in maintaining the caveola membrane domain and modulates the interaction of GPI-anchored membrane proteins via their phospholipid anchors.

MeSH Terms
Animals Carrier Proteins/metabolism Cholesterol/physiology Filipin/pharmacology Folate Receptors, GPI-Anchored Glycolipids/metabolism Glycosylphosphatidylinositols In Vitro Techniques Phosphatidylinositols/metabolism Receptor Aggregation/drug effects,physiology Receptors, Cell Surface Tetrahydrofolates/metabolism
Chemicals
Carrier Proteins Folate Receptors, GPI-Anchored Glycolipids Glycosylphosphatidylinositols Phosphatidylinositols Receptors, Cell Surface Tetrahydrofolates Filipin Cholesterol 5-methyltetrahydrofolate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rothberg K G
Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas 75235.
Ying Y S
Kamen B A
Anderson R G
References (40)
40 references, click to expand
  1. Induction of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in human fibroblasts incubated with compactin (ML-236B), a competitive inhibitor of the reductase.
    J Biol Chem. 1978 Feb 25;253(4):1121-8 PMID: 624722
  2. Glycophospholipid membrane anchoring provides clues to the mechanism of protein sorting in polarized epithelial cells.
    Trends Biochem Sci. 1990 Mar;15(3):113-8 PMID: 2139258
  3. Procedure for determination of free and total cholesterol in micro- or nanogram amounts suitable for studies with cultured cells.
    J Lipid Res. 1978 Nov;19(8):1068-70 PMID: 731127
  4. Anionic lipid domains: correlation with functional topography in a mammalian cell membrane.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6601-5 PMID: 6935671
  5. Heterogeneous distribution of filipin--cholesterol complexes across the cisternae of the Golgi apparatus.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):293-7 PMID: 7017713
  6. Isolation and characterization of a folate receptor from human placenta.
    J Biol Chem. 1981 Sep 25;256(18):9684-92 PMID: 6169714
  7. The activity of 5'-nucleotidase in liver plasma membranes is affected by the increase in bilayer fluidity achieved by anionic drugs but not by cationic drugs.
    J Biol Chem. 1982 Feb 25;257(4):1811-5 PMID: 6276399
  8. Non-coated membrane invaginations are involved in binding and internalization of cholera and tetanus toxins.
    Nature. 1982 Apr 15;296(5858):651-3 PMID: 7070509
  9. Modifications of anionic-lipid domains preceding membrane fusion in guinea pig sperm.
    J Cell Biol. 1982 Mar;92(3):604-15 PMID: 7085750
  10. Filipin-cholesterol complexes form in uncoated vesicle membrane derived from coated vesicles during receptor-mediated endocytosis of low density lipoprotein.
    J Cell Biol. 1983 May;96(5):1273-8 PMID: 6132922
  11. Detection of microdomains in biomembranes. An appraisal of recent developments in freeze-fracture cytochemistry.
    Biochim Biophys Acta. 1983 Aug 11;737(3-4):373-408 PMID: 6349687
  12. Rings of membrane sterols surround the openings of vesicles and fenestrae, in capillary endothelium.
    J Cell Biol. 1983 Nov;97(5 Pt 1):1592-600 PMID: 6415070
  13. Ultrastructural analysis of crystalloid endoplasmic reticulum in UT-1 cells and its disappearance in response to cholesterol.
    J Cell Sci. 1983 Sep;63:1-20 PMID: 6685129
  14. Receptor-mediated endocytosis of low-density lipoprotein in cultured cells.
    Methods Enzymol. 1983;98:241-60 PMID: 6321901
  15. Association of the postsynaptic 43K protein with newly formed acetylcholine receptor clusters in cultured muscle cells.
    J Cell Biol. 1985 May;100(5):1698-705 PMID: 3886673
  16. Cholesterol and the cell membrane.
    Biochim Biophys Acta. 1985 Dec 9;822(3-4):267-87 PMID: 3904832
  17. Clustered distribution of human placental alkaline phosphatase on the surface of both placental and cancer cells. Electron microscopic observations using gold-labeled antibodies.
    J Histochem Cytochem. 1985 Dec;33(12):1227-34 PMID: 4067276
  18. Methods for visualization of the LDL pathway in cultured human fibroblasts.
    Methods Enzymol. 1986;129:201-16 PMID: 3523145
  19. Effects of nystatin on intracellular contents and membrane transport of alkali cations, and cell volume in HeLa cells.
    J Membr Biol. 1986;90(3):231-40 PMID: 3735403
  20. How do the polyene macrolide antibiotics affect the cellular membrane properties?
    Biochim Biophys Acta. 1986 Dec 22;864(3-4):257-304 PMID: 3539192
  21. Acetylcholine receptor clustering and nuclear movement in muscle fibers in culture.
    J Cell Biol. 1987 Jan;104(1):87-95 PMID: 3793762
  22. Receptor-mediated endocytosis: concepts emerging from the LDL receptor system.
    Annu Rev Cell Biol. 1985;1:1-39 PMID: 2881559
  23. Progress in unraveling pathways of Golgi traffic.
    Annu Rev Cell Biol. 1985;1:447-88 PMID: 3916320
  24. Sorting of sphingolipids in epithelial (Madin-Darby canine kidney) cells.
    J Cell Biol. 1987 Oct;105(4):1623-35 PMID: 3667693
  25. Ligands internalized through coated or noncoated invaginations follow a common intracellular pathway.
    Proc Natl Acad Sci U S A. 1987 Nov;84(22):7957-61 PMID: 2446314
  26. Identification of agrin, a synaptic organizing protein from Torpedo electric organ.
    J Cell Biol. 1987 Dec;105(6 Pt 1):2471-8 PMID: 2826489
  27. The lateral distribution of cholesterol in the plane of lipid multibilayers.
    Biochim Biophys Acta. 1988 Jan 22;937(2):417-21 PMID: 3337810
  28. Orthogonal arrays of particles in non-pigmented cells of rat ciliary epithelium: relation to distribution of filipin- and digitonin-induced alterations of the basolateral membrane.
    Cell Tissue Res. 1988 Apr;252(1):165-73 PMID: 3378258
  29. Increase in membrane fluidity and opening of tight junctions have similar effects on sodium-coupled uptakes in renal epithelial cells.
    J Biol Chem. 1988 Aug 15;263(23):11183-8 PMID: 3403520
  30. Delivery of folates to the cytoplasm of MA104 cells is mediated by a surface membrane receptor that recycles.
    J Biol Chem. 1988 Sep 25;263(27):13602-9 PMID: 3417674
  31. Cell-surface anchoring of proteins via glycosyl-phosphatidylinositol structures.
    Annu Rev Biochem. 1988;57:285-320 PMID: 3052274
  32. Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton.
    Annu Rev Cell Biol. 1988;4:487-525 PMID: 3058164
  33. Polarized apical distribution of glycosyl-phosphatidylinositol-anchored proteins in a renal epithelial cell line.
    Proc Natl Acad Sci U S A. 1988 Dec;85(24):9557-61 PMID: 2974157
  34. Mechanism of membrane anchoring affects polarized expression of two proteins in MDCK cells.
    Science. 1989 Sep 29;245(4925):1499-501 PMID: 2571189
  35. A glycophospholipid membrane anchor acts as an apical targeting signal in polarized epithelial cells.
    J Cell Biol. 1989 Nov;109(5):2145-56 PMID: 2478564
  36. Lipid traffic in animal cells.
    Annu Rev Cell Biol. 1989;5:247-75 PMID: 2688705
  37. A human membrane-associated folate binding protein is anchored by a glycosyl-phosphatidylinositol tail.
    J Biol Chem. 1989 Dec 25;264(36):21446-9 PMID: 2557328
  38. The glycophospholipid-linked folate receptor internalizes folate without entering the clathrin-coated pit endocytic pathway.
    J Cell Biol. 1990 Mar;110(3):637-49 PMID: 1968465
  39. The molecular constituents of intercellular junctions.
    Curr Opin Cell Biol. 1989 Oct;1(5):884-91 PMID: 2560659
  40. Freeze-fracture identification of sterol-digitonin complexes in cell and liposome membranes.
    J Cell Biol. 1978 Aug;78(2):577-96 PMID: 690180
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.111.6.2931
Published
1990-12-00
Pages
2931-8
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116385
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.111.6.2931 PMC Full Text View journal details
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