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PMID: 2156800 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Activation of the lac genes of Tn951 by insertion sequences from Pseudomonas cepacia.

Journal of bacteriology ·Vol. 172 ·No. 4 ·1990-04-00 ·Pages 1719-24

Wood MS, Lory C, Lessie TG

Abstract

We have identified three transposable gene-activating elements from Pseudomonas cepacia on the basis of their abilities to increase expression of the lac genes of the broad-host-range plasmid pGC91.14 (pRP1::Tn951). When introduced into auxotrophic derivatives of P. cepacia 249 (ATCC 17616), this plasmid failed to confer the ability to utilize lactose. The lac genes of Tn951 were poorly expressed in P. cepacia and were not induced by isopropyl-beta-D-thiogalactopyranoside. Lac+ variants of the pGC91.14-containing strains which formed beta-galactosidase at high constitutive levels as a consequence of transposition of insertion sequences from the P. cepacia genome to sites upstream of the lacZ gene of Tn951 were isolated. Certain of the elements also increased gene expression in other bacteria. For example, IS407 strongly activated the lacZ gene of Tn951 in Pseudomonas aeruginosa and Escherichia coli, and IS406 (but not IS407) did so in Zymomonas mobilis. The results indicate that IS elements from P. cepacia have potential for turning on the expression of foreign genes in a variety of gram-negative bacteria.

MeSH Terms
Blotting, Southern DNA Transposable Elements Escherichia coli/genetics Galactosidases/genetics Gene Expression Regulation, Bacterial Genes, Bacterial Nucleic Acid Hybridization Plasmids Pseudomonas/genetics RNA, Messenger/genetics Restriction Mapping Transcription, Genetic beta-Galactosidase/genetics
Chemicals
DNA Transposable Elements RNA, Messenger Galactosidases beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wood M S
Department of Microbiology, University of Massachusetts, Amherst 01003.
Lory C
Lessie T G
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26 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.4.1719-1724.1990
Published
1990-04-00
Pages
1719-24
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208661
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.4.1719-1724.1990 PMC Full Text View journal details
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