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PMID: 2159623 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The primary structure and expression of the second open reading frame of the polymerase gene of the coronavirus MHV-A59; a highly conserved polymerase is expressed by an efficient ribosomal frameshifting mechanism.

Nucleic acids research ·Vol. 18 ·No. 7 ·1990-04-11 ·Pages 1825-32

Bredenbeek PJ, Pachuk CJ, Noten AF, Charité J, Luytjes W, Weiss SR, Spaan WJ

Abstract

Sequence analysis of a substantial part of the polymerase gene of the murine coronavirus MHV-A59 revealed the 3' end of an open reading frame (ORF1a) overlapping with a large ORF (ORF1b; 2733 amino acids) which covers the 3' half of the polymerase gene. The expression of ORF1b occurs by a ribosomal frameshifting mechanism since the ORF1a/ORF1b overlapping nucleotide sequence is capable of inducing ribosomal frameshifting in vitro as well as in vivo. A stem-loop structure and a pseudoknot are predicted in the nucleotide sequence involved in ribosomal frameshifting. Comparison of the predicted amino acid sequence of MHV ORF1b with the amino acid sequence deduced from the corresponding gene of the avian coronavirus IBV demonstrated that in contrast to the other viral genes this ORF is extremely conserved. Detailed analysis of the predicted amino acid sequence revealed sequence elements which are conserved in many DNA and RNA polymerases.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular DNA-Directed RNA Polymerases/genetics Gene Expression Genes, Viral Mice Molecular Sequence Data Murine hepatitis virus/enzymology,genetics Nucleic Acid Conformation Oligonucleotide Probes RNA, Viral/genetics,isolation & purification Restriction Mapping Ribosomes/metabolism
Chemicals
Oligonucleotide Probes RNA, Viral DNA-Directed RNA Polymerases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Bredenbeek P J
Department of Infectious Diseases and Immunology, State University of Utrecht, The Netherlands.
Pachuk C J
Noten A F
Charité J
Luytjes W
Weiss S R
Spaan W J
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.7.1825
Published
1990-04-11
Pages
1825-32
Language
English
Region
England
NLM ID
0411011
PMCID
PMC330602
Subset
IM
Grants
PHS HHS · A1-17418 · United States
NINDS NIH HHS · NS-21954 · United States
PHS HHS · T32 A1-07325 · United States
Databases
GENBANK
X51939
External Links
PubMed source DOI: 10.1093/nar/18.7.1825 PMC Full Text View journal details
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