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PMID: 2165605 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Oxidation-reduction sensitive interaction of a cellular 50-kDa protein with an RNA hairpin in the 5' noncoding region of the poliovirus genome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 15 ·1990-08-00 ·Pages 5846-50

Najita L, Sarnow P

Abstract

Genetic and biochemical analyses of the 5' noncoding region of poliovirus have indicated the importance of this region in both translation and amplification of the viral RNA. The role of the cellular machinery required for these events is just beginning to be revealed. Using an RNA gel retention assay, we have identified a cellular 50-kDa protein that forms a specific complex with a stable stem-loop structure present in the viral 5' noncoding region. The formation of the RNA-protein complex is dependent on the availability of free sulfhydryl groups in the protein. The possible involvement of this RNA-protein complex in the regulation of viral gene expression is discussed.

MeSH Terms
Base Sequence Binding Sites Cell Transformation, Viral Genes, Viral HeLa Cells/metabolism Humans Molecular Sequence Data Molecular Weight Neoplasm Proteins/isolation & purification,metabolism Nucleic Acid Conformation Plasmids Poliovirus/genetics,metabolism RNA, Viral/genetics,metabolism
Chemicals
Neoplasm Proteins RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Najita L
Department of Microbiology and Immunology, University of Colorado Health Sciences Center, Denver 80262.
Sarnow P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.15.5846
Published
1990-08-00
Pages
5846-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54425
Subset
IM
Grants
NIA NIH HHS · AG-07347 · United States
NIAID NIH HHS · AI-25105 · United States
NINDS NIH HHS · NS-07321 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.15.5846 PMC Full Text View journal details
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