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PMID: 2168560 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Allele loss on chromosome 16 associated with progression of human hepatocellular carcinoma.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 17 ·1990-09-00 ·Pages 6791-4

Tsuda H, Zhang WD, Shimosato Y, Yokota J, Terada M, Sugimura T, Miyamura T, Hirohashi S

Abstract

Loss of heterozygosity on chromosome 16 is a common genetic alteration in human hepatocellular carcinoma (HCC). To clarify the pathogenetic significance of allele loss on chromosome 16, we performed restriction fragment length polymorphism analysis of 70 surgically resected tumors by using 15 polymorphic DNA markers for chromosome 16. Loss of heterozygosity on chromosome 16 was detected in 36 (52%) of 69 informative cases, and the common region of allele loss in these 36 tumors was located between the HP locus (16q22.1) and the CTRB locus (16q22.3-q23.2). These losses occurred more frequently in HCCs of poor differentiation, of larger size, and with metastasis, whereas they were not detected in HCC at the earliest stage. In addition, these losses were not associated with presence or absence of hepatitis B virus DNA integration or hepatitis C virus infection. These results show that loss of heterozygosity on chromosome 16 is a late event occurring after hepatocarcinogenesis and strongly suggest that this phenomenon is involved in enhancement of tumor aggressiveness during progression of HCC.

MeSH Terms
Alleles Carcinoma, Hepatocellular/genetics,pathology Chromosome Deletion Chromosome Mapping Chromosomes, Human, Pair 16 DNA Probes DNA, Viral/isolation & purification Hepatitis B virus/isolation & purification Heterozygote Humans Liver/microbiology,pathology Liver Cirrhosis/pathology Liver Neoplasms/genetics,pathology Liver Regeneration Models, Biological
Chemicals
DNA Probes DNA, Viral
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Tsuda H
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan.
Zhang W D
Shimosato Y
Yokota J
Terada M
Sugimura T
Miyamura T
Hirohashi S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.17.6791
Published
1990-09-00
Pages
6791-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54623
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.87.17.6791 PMC Full Text View journal details
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