Home LiteratureArticle Details
PMID: 2168848 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Structural genes for nitrate-inducible formate dehydrogenase in Escherichia coli K-12.

Genetics ·Vol. 125 ·No. 4 ·1990-08-00 ·Pages 691-702

Berg BL, Stewart V

Abstract

Formate oxidation coupled to nitrate reduction constitutes a major anaerobic respiratory pathway in Escherichia coli. This respiratory chain consists of formate dehydrogenase-N, quinone, and nitrate reductase. We have isolated a recombinant DNA clone that likely contains the structural genes, fdnGHI, for the three subunits of formate dehydrogenase-N. The fdnGHI clone produced proteins of 110, 32 and 20 kDa which correspond to the subunit sizes of purified formate dehydrogenase-N. Our analysis indicates that fdnGHI is organized as an operon. We mapped the fdn operon to 32 min on the E. coli genetic map, close to the genes for cryptic nitrate reductase (encoded by the narZ operon). Expression of phi(fdnG-lacZ) operon fusions was induced by anaerobiosis and nitrate. This induction required fnr+ and narL+, two regulatory genes whose products are also required for the anaerobic, nitrate-inducible activation of the nitrate reductase structural gene operon, narGHJI. We conclude that regulation of fdnGHI and narGHJI expression is mediated through common pathways.

MeSH Terms
Aldehyde Oxidoreductases/genetics Cloning, Molecular DNA Transposable Elements Enzyme Induction Escherichia coli/enzymology,genetics Escherichia coli Proteins Formate Dehydrogenases/biosynthesis,genetics Gene Expression Regulation, Bacterial Genes, Bacterial Genes, Regulator Models, Genetic Mutation Nitrate Reductase Nitrate Reductases/genetics Nitrates/pharmacology Operon Oxidation-Reduction Restriction Mapping
Chemicals
DNA Transposable Elements Escherichia coli Proteins Nitrates Formate Dehydrogenases Aldehyde Oxidoreductases Nitrate Reductases Nitrate Reductase narZ protein, E coli
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Berg B L
Section of Microbiology, Cornell University, Ithaca, New York 14853-8101.
Stewart V
References (50)
50 references, click to expand
  1. Nucleotide sequence of the narL gene that is involved in global regulation of nitrate controlled respiratory genes of Escherichia coli.
    Nucleic Acids Res. 1989 Mar 11;17(5):1965-75 PMID: 2648330
  2. Formic dehydrogenase and the hydrogenlyase enzyme complex in coli-aerogenes bacteria.
    J Bacteriol. 1957 Jun;73(6):706-21 PMID: 13449036
  3. Occurrence in vivo of selenocysteyl-tRNA(SERUCA) in Escherichia coli. Effect of sel mutations.
    J Biol Chem. 1989 Jun 15;264(17):9720-3 PMID: 2524495
  4. The narX and narL genes encoding the nitrate-sensing regulators of Escherichia coli are homologous to a family of prokaryotic two-component regulatory genes.
    Nucleic Acids Res. 1989 Apr 25;17(8):2947-57 PMID: 2657652
  5. Cloning of binding sequences for the Escherichia coli transcription activators, FNR and CRP: location of bases involved in discrimination between FNR and CRP.
    Nucleic Acids Res. 1989 May 25;17(10):3865-74 PMID: 2543955
  6. Specificity of transposon Tn5 insertion.
    Genetics. 1983 Dec;105(4):813-28 PMID: 6315531
  7. In vitro insertional mutagenesis with a selectable DNA fragment.
    Gene. 1984 Sep;29(3):303-13 PMID: 6237955
  8. Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli.
    J Bacteriol. 1985 Mar;161(3):1219-21 PMID: 2982787
  9. The seleno-polypeptide of formic dehydrogenase (formate hydrogen-lyase linked) from Escherichia coli: genetic analysis.
    Arch Microbiol. 1985 May;141(4):359-63 PMID: 3160320
  10. Isolation, characterization and complementation analysis of nirB mutants of Escherichia coli deficient only in NADH-dependent nitrite reductase activity.
    J Gen Microbiol. 1985 Oct;131(10):2771-82 PMID: 3906030
  11. pHG165: a pBR322 copy number derivative of pUC8 for cloning and expression.
    Plasmid. 1986 May;15(3):172-81 PMID: 3012611
  12. Mini-mu bacteriophage with plasmid replicons for in vivo cloning and lac gene fusing.
    J Bacteriol. 1986 Oct;168(1):357-64 PMID: 3020001
  13. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.
    J Mol Biol. 1986 May 5;189(1):113-30 PMID: 3537305
  14. Cotranslational insertion of selenocysteine into formate dehydrogenase from Escherichia coli directed by a UGA codon.
    Proc Natl Acad Sci U S A. 1987 May;84(10):3156-60 PMID: 3033637
  15. The narL gene product activates the nitrate reductase operon and represses the fumarate reductase and trimethylamine N-oxide reductase operons in Escherichia coli.
    Proc Natl Acad Sci U S A. 1987 Jun;84(11):3901-5 PMID: 3035558
  16. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.
    Cell. 1987 Jul 31;50(3):495-508 PMID: 3038334
  17. Factors affecting transcriptional regulation of the formate-hydrogen-lyase pathway of Escherichia coli.
    Arch Microbiol. 1987 Jun;148(1):44-51 PMID: 2443100
  18. Formate-nitrate respiration in Salmonella typhimurium: studies of two rha-linked fdn genes.
    J Bacteriol. 1988 Jan;170(1):213-7 PMID: 3275611
  19. Nitrate reductase complex of Escherichia coli K-12: participation of specific formate dehydrogenase and cytochrome b1 components in nitrate reduction.
    J Bacteriol. 1969 Sep;99(3):720-9 PMID: 4905536
  20. Effects of molybdate and selenite on formate and nitrate metabolism in Escherichia coli.
    J Bacteriol. 1971 Mar;105(3):1006-14 PMID: 4926673
  21. The role of a novel cytochrome b-containing nitrate reductase and quinone in the in vitro reconstruction of formate-nitrate reductase activity of E. coli.
    Biochem Biophys Res Commun. 1974 Dec 23;61(4):1234-41 PMID: 4616697
  22. The purification and properties of formate dehydrogenase and nitrate reductase from Escherichia coli.
    J Biol Chem. 1975 Sep 10;250(17):6693-705 PMID: 1099093
  23. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  24. Mutants of Escherichia coli K12 unable to use fumarate as an anaerobic electron acceptor.
    J Gen Microbiol. 1976 Dec;97(2):145-60 PMID: 796407
  25. Anaerobic growth of Escherichia coli on formate by reduction of nitrate, fumarate, and trimethylamine N-oxide.
    Z Allg Mikrobiol. 1977;17(3):235-42 PMID: 327708
  26. Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics.
    J Mol Biol. 1977 Oct 15;116(1):125-59 PMID: 338917
  27. The chromosomal location and pleiotropic effects of mutations of the nirA+ gene of Escherichia coli K12: the essential role of nirA+ in nitrite reduction and in other anaerobic redox reactions.
    J Gen Microbiol. 1978 May;106(1):1-12 PMID: 206651
  28. Formate-nitrite reduction in Escherchia coli K12. 1. Physiological study of the system.
    Eur J Biochem. 1979 Apr 2;95(2):309-14 PMID: 37075
  29. Formate-nitrite reduction in Escherichia coli K12. 2. Identification of components involved in the electron transfer.
    Eur J Biochem. 1979 Apr 2;95(2):315-21 PMID: 378660
  30. Primary structure of a chloramphenicol acetyltransferase specified by R plasmids.
    Nature. 1979 Dec 20-27;282(5741):870-2 PMID: 390404
  31. Formate dehydrogenase mutants of Salmonella typhimurium: a new medium for their isolation and new mutant classes.
    Mol Gen Genet. 1979;177(1):95-101 PMID: 395418
  32. The inverted repeats of Tn5 are functionally different.
    Cell. 1980 Mar;19(3):795-805 PMID: 6244898
  33. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.
    Anal Biochem. 1979 Sep 15;98(1):132-5 PMID: 543547
  34. Resolution of distinct selenium-containing formate dehydrogenases from Escherichia coli.
    J Bacteriol. 1981 Mar;145(3):1317-24 PMID: 7009577
  35. Salmonella typhimurium mutants defective in the formate dehydrogenase linked to nitrate reductase.
    J Bacteriol. 1982 Feb;149(2):554-60 PMID: 7035433
  36. Generation of a membrane potential by one of two independent pathways for nitrite reduction by Escherichia coli.
    J Gen Microbiol. 1982 Jan;128(1):219-22 PMID: 6283015
  37. Nitrate reductase in Escherichia coli K-12: involvement of chlC, chlE, and chlG loci.
    J Bacteriol. 1982 Aug;151(2):788-99 PMID: 7047497
  38. Amplification and product identification of the fnr gene of Escherichia coli.
    J Gen Microbiol. 1982 Oct;128(10):2221-8 PMID: 6759613
  39. Escherichia coli genes whose products are involved in selenium metabolism.
    J Bacteriol. 1988 Feb;170(2):540-6 PMID: 2962989
  40. Involvement of the ntrA gene product in the anaerobic metabolism of Escherichia coli.
    Mol Gen Genet. 1987 Dec;210(3):535-42 PMID: 3323848
  41. arcA (dye), a global regulatory gene in Escherichia coli mediating repression of enzymes in aerobic pathways.
    Proc Natl Acad Sci U S A. 1988 Mar;85(6):1888-92 PMID: 2964639
  42. Polarity effects in the hisG gene of salmonella require a site within the coding sequence.
    Genetics. 1988 Feb;118(2):193-202 PMID: 3282985
  43. Location of sequences in the nar promoter of Escherichia coli required for regulation by Fnr and NarL.
    J Biol Chem. 1988 Sep 25;263(27):13700-5 PMID: 3138237
  44. Influence of nar (nitrate reductase) genes on nitrate inhibition of formate-hydrogen lyase and fumarate reductase gene expression in Escherichia coli K-12.
    J Bacteriol. 1988 Oct;170(10):4437-44 PMID: 3049531
  45. Identification of a second gene involved in global regulation of fumarate reductase and other nitrate-controlled genes for anaerobic respiration in Escherichia coli.
    J Bacteriol. 1989 Jul;171(7):3810-6 PMID: 2544557
  46. Expression of sigma 54 (ntrA)-dependent genes is probably united by a common mechanism.
    Microbiol Rev. 1989 Sep;53(3):367-76 PMID: 2677638
  47. Mutants of Escherichia coli specifically deficient in respiratory formate dehydrogenase activity.
    J Gen Microbiol. 1988 Dec;134(12):3129-39 PMID: 3077634
  48. Identification of a novel translation factor necessary for the incorporation of selenocysteine into protein.
    Nature. 1989 Nov 23;342(6248):453-6 PMID: 2531290
  49. Protein phosphorylation and regulation of adaptive responses in bacteria.
    Microbiol Rev. 1989 Dec;53(4):450-90 PMID: 2556636
  50. Structure of genes narL and narX of the nar (nitrate reductase) locus in Escherichia coli K-12.
    J Bacteriol. 1989 Apr;171(4):2229-34 PMID: 2649492
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/125.4.691
Published
1990-08-00
Pages
691-702
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1204095
Subset
IM
Grants
NIGMS NIH HHS · GM36877 · United States
External Links
PubMed source DOI: 10.1093/genetics/125.4.691 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2