Home LiteratureArticle Details
PMID: 2169872 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Alpha 1 beta 1 integrin heterodimer functions as a dual laminin/collagen receptor in neural cells.

Biochemistry ·Vol. 29 ·No. 27 ·1990-07-10 ·Pages 6540-4

Tawil NJ, Houde M, Blacher R, Esch F, Reichardt LF, Turner DC, Carbonetto S

Abstract

A monoclonal antibody (3A3) raised against a rat neural cell line (PC12) was shown previously to bind to the surfaces of these cells, inhibiting substratum adhesion. Immunochemical and other data indicated that the heterodimer recognized by 3A3 was a member of the integrin family of adhesive receptors and had a beta 1 subunit. The relationship of the alpha subunit to other integrins was unknown. Here we show that 3A3 recognizes in rat tissues a heterodimer (approximately 185 kDa, approximately 110 kDa; unreduced) that is electrophoretically and immunochemically indistinguishable from the antigen in PC12 cells. Immunoaffinity purification of the heterodimer from neonatal rats and protein microsequencing indicate that the alpha subunit is identical at 11 or 13 N-terminal residues with VLA-1, an integrin on human hematopoietic cells. Monoclonal antibody 3A3 inhibits the attachment of rat astrocytes to laminin or collagen but not to fibronectin or polylysine. These data suggest strongly that the integrin recognized by 3A3 is the rat homologue of VLA-1, i.e., alpha 1 beta 1, and that alpha 1 beta 1 is a dual laminin/collagen receptor.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Monoclonal/immunology Astrocytes/metabolism Cell Adhesion/drug effects Cell Line Collagen/metabolism Humans Laminin/metabolism Molecular Sequence Data Neurons/chemistry Rats Receptors, Cell Surface/immunology,isolation & purification Receptors, Collagen Receptors, Immunologic/immunology,isolation & purification Receptors, Laminin Receptors, Very Late Antigen/genetics Sequence Homology, Nucleic Acid
Chemicals
Antibodies, Monoclonal Laminin Receptors, Cell Surface Receptors, Collagen Receptors, Immunologic Receptors, Laminin Receptors, Very Late Antigen Collagen
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Tawil N J
Centre for Research in Neuroscience, McGill University, Montreal General Hospital Research Institute, Quebec, Canada.
Houde M
Blacher R
Esch F
Reichardt L F
Turner D C
Carbonetto S
References (28)
28 references, click to expand
  1. Integrin heterodimer and receptor complexity in avian and mammalian cells.
    J Cell Biol. 1989 Jul;109(1):409-20 PMID: 2663881
  2. Integrins: a family of cell surface receptors.
    Cell. 1987 Feb 27;48(4):549-54 PMID: 3028640
  3. Fibronectin mediates attachment of chicken myoblasts to a gelatin-coated substratum.
    J Biol Chem. 1979 Jun 25;254(12):5475-82 PMID: 109435
  4. The fibronectin receptor family.
    Nature. 1986 Jun 19-25;321(6072):728 PMID: 2940466
  5. Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits.
    J Cell Biol. 1987 Oct;105(4):1873-84 PMID: 2822727
  6. Identification of a neuronal laminin receptor: an Mr 200K/120K integrin heterodimer that binds laminin in a divalent cation-dependent manner.
    Neuron. 1988 Oct;1(8):713-25 PMID: 2978786
  7. Cell surface receptors for extracellular matrix molecules.
    Annu Rev Cell Biol. 1987;3:179-205 PMID: 2825736
  8. Adhesive protein receptors on hematopoietic cells.
    Immunol Today. 1988 Apr;9(4):109-13 PMID: 2855703
  9. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  10. Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue.
    J Cell Biol. 1980 Jun;85(3):890-902 PMID: 6248568
  11. Isolation of acetylcholine receptor clusters in substrate-associated material from cultured rat myotubes using saponin.
    J Cell Biol. 1984 Sep;99(3):984-93 PMID: 6381511
  12. Analysis of phenylthiohydantoins by ultrasensitive gradient high-performance liquid chromatography.
    Methods Enzymol. 1983;91:486-93 PMID: 6855596
  13. Interactions of a neuronal cell line (PC12) with laminin, collagen IV, and fibronectin: identification of integrin-related glycoproteins involved in attachment and process outgrowth.
    J Cell Biol. 1987 Nov;105(5):2347-58 PMID: 3500175
  14. The very late antigen family of heterodimers is part of a superfamily of molecules involved in adhesion and embryogenesis.
    Proc Natl Acad Sci U S A. 1987 May;84(10):3239-43 PMID: 3033641
  15. Identification of integrin collagen receptors on human melanoma cells.
    J Biol Chem. 1989 Mar 15;264(8):4684-8 PMID: 2538453
  16. Dispersal and reformation of acetylcholine receptor clusters of cultured rat myotubes treated with inhibitors of energy metabolism.
    J Cell Biol. 1979 Sep;82(3):626-43 PMID: 511929
  17. Magnesium-dependent attachment and neurite outgrowth by PC12 cells on collagen and laminin substrata.
    Dev Biol. 1987 Jun;121(2):510-25 PMID: 3582737
  18. The VLA protein family. Characterization of five distinct cell surface heterodimers each with a common 130,000 molecular weight beta subunit.
    J Biol Chem. 1987 Mar 5;262(7):3300-9 PMID: 3546305
  19. New perspectives in cell adhesion: RGD and integrins.
    Science. 1987 Oct 23;238(4826):491-7 PMID: 2821619
  20. A novel vitronectin receptor integrin (alpha v beta x) is responsible for distinct adhesive properties of carcinoma cells.
    Cell. 1989 Apr 7;57(1):59-69 PMID: 2467745
  21. Comparison of fibronectin receptors from rat hepatocytes and fibroblasts.
    J Biol Chem. 1987 Jun 5;262(16):7819-24 PMID: 2953726
  22. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.
    J Biol Chem. 1987 Jul 25;262(21):10035-8 PMID: 3611052
  23. Laminin.
    Methods Enzymol. 1982;82 Pt A:831-8 PMID: 7078458
  24. A dual laminin/collagen receptor acts in peripheral nerve regeneration.
    Proc Natl Acad Sci U S A. 1990 Feb;87(4):1319-22 PMID: 2154740
  25. Identification of a cell-surface protein involved in PC12 cell-substratum adhesion and neurite outgrowth on laminin and collagen.
    J Neurosci. 1989 Sep;9(9):3287-96 PMID: 2552042
  26. Fibronectin receptor structures in the VLA family of heterodimers.
    Nature. 1987 Apr 9-15;326(6113):607-9 PMID: 3031508
  27. Purification and characterization of mammalian integrins expressed by a rat neuronal cell line (PC12): evidence that they function as alpha/beta heterodimeric receptors for laminin and type IV collagen.
    J Cell Biol. 1988 Sep;107(3):1241-52 PMID: 2843550
  28. The alpha 1/beta 1 and alpha 6/beta 1 integrin heterodimers mediate cell attachment to distinct sites on laminin.
    J Cell Biol. 1990 Jun;110(6):2175-84 PMID: 2351695
Article Info
Journal
Biochemistry
Abbr.
Biochemistry
ISSN
0006-2960
DOI
10.1021/bi00479a028
Published
1990-07-10
Pages
6540-4
Language
English
Region
United States
NLM ID
0370623
PMCID
PMC2710132
Subset
IM
Grants
NINDS NIH HHS · R01 NS019090 · United States
NINDS NIH HHS · R01 NS019090-14 · United States
NINDS NIH HHS · NS 27409 · United States
External Links
PubMed source DOI: 10.1021/bi00479a028 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2