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PMID: 216996 Published · ppublish English Journal Article

Sequence of a RNA templated by the 3'-OH RNA terminus of defective interfering particles of vesicular stomatitis virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 10 ·1978-10-00 ·Pages 4704-8

Semler BL, Perrault J, Abelson J, Holland JJ

Abstract

We have sequenced the endogenous RNA polymerase product produced by disrupted purified virions of vesicular stomatitis virus defective interfering particles by using the newer one-dimensional rapid gel sequencing techniques and confirming this with a modified two-dimensional gel vectoring technique. The sequence of this 46-nucleotide RNA is: 5'(pp)pACGAAGACCACAAAACCA-GAUAAAAAAUAAAAACCACAAGAGGG(U)COH3'. We infer that this sequence is identical to the sequence at the 5' end of infectious vesicular stomatitis virus RNA and is complementary to the sequence of the 3'-OH terminus of this defective interfering particle genome RNA.

MeSH Terms
Base Sequence DNA-Directed RNA Polymerases/metabolism Defective Viruses/metabolism RNA, Viral/metabolism Templates, Genetic Vesicular stomatitis Indiana virus/metabolism
Chemicals
RNA, Viral DNA-Directed RNA Polymerases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Semler B L
Perrault J
Abelson J
Holland J J
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.10.4704
Published
1978-10-00
Pages
4704-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC336188
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.10.4704 PMC Full Text View journal details
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