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PMID: 2174165 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Epstein-Barr virus small nuclear RNAs are not expressed in permissively infected cells in AIDS-associated leukoplakia.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 22 ·1990-11-00 ·Pages 8790-4

Gilligan K, Rajadurai P, Resnick L, Raab-Traub N

Abstract

Epstein-Barr virus (EBV) DNA structure and gene expression were analyzed in tissue specimens from oral hairy leukoplakia (HLP), a mucocutaneous lesion that develops in patients infected with human immunodeficiency virus (HIV). The structure of the terminal restriction enzyme fragments of EBV revealed that HLP is a permissive infection without a predominant, detectable population of EBV episomal DNA. In RNA preparations from this uniquely permissive infection, EBV replicative mRNAs could be identified by Northern analysis; however, the virally encoded small nuclear RNAs, the EBERs, were not detected in most HLP RNA preparations. In situ hybridization detected EBER expression in very rare cells. These data indicate that unlike other viral small nuclear RNAs, the EBERs are not expressed during viral replication and must participate in the complex maintenance of latent EBV infection.

MeSH Terms
Acquired Immunodeficiency Syndrome/microbiology Blotting, Southern Gene Expression Regulation, Viral Herpesviridae Infections/genetics Herpesvirus 4, Human/genetics Humans Leukoplakia/microbiology Nucleic Acid Hybridization RNA Probes RNA, Small Nuclear/genetics RNA, Viral/genetics Transcription, Genetic Virus Replication
Chemicals
RNA Probes RNA, Small Nuclear RNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gilligan K
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599.
Rajadurai P
Resnick L
Raab-Traub N
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.22.8790
Published
1990-11-00
Pages
8790-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC55045
Subset
IM
Grants
NCI NIH HHS · CA32979 · United States
NCI NIH HHS · CA52406 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.22.8790 PMC Full Text View journal details
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