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PMID: 2181259 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S. Review

Uncoupler-resistant mutants of bacteria.

Microbiological reviews ·Vol. 54 ·No. 1 ·1990-03-00 ·Pages 52-65

Krulwich TA, Quirk PG, Guffanti AA

Abstract

The chemiosmotic model of energy transduction offers a satisfying and widely confirmed understanding of the action of uncouplers on such processes as oxidative phosphorylation; the uncoupler, by facilitating the transmembrane movement of protons or other compensatory ions, reduces the electrochemical proton gradient that is posited as the energy intermediate for many kinds of bioenergetic work. In connection with this formulation, uncoupler-resistant mutants of bacteria that neither exclude nor inactivate these agents represent a bioenergetic puzzle. Uncoupler-resistant mutants of aerobic Bacillus species are, in fact, membrane lipid mutants with bioenergetic properties that are indeed challenging in connection with the chemiosmotic model. By contrast, uncoupler-resistant mutants of Escherichia coli probably exclude uncouplers, sometimes only under rather specific conditions. Related phenomena in eucaryotic and procaryotic systems, as well as various observations on uncouplers, decouplers, and certain other membrane-active agents, are also briefly considered.

MeSH Terms
Bacillus/drug effects,genetics Drug Resistance, Microbial/genetics Escherichia coli/drug effects,genetics Mutation Oxidative Phosphorylation/drug effects Uncoupling Agents/pharmacology
Chemicals
Uncoupling Agents
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Krulwich T A
Department of Biochemistry, Mount Sinai School of Medicine, City University of New York, New York 10029.
Quirk P G
Guffanti A A
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Article Info
Journal
Microbiological reviews
Abbr.
Microbiol Rev
ISSN
0146-0749
DOI
10.1128/mr.54.1.52-65.1990
Published
1990-03-00
Pages
52-65
Language
English
Region
United States
NLM ID
7806086
PMCID
PMC372758
Subset
IM
Grants
NIGMS NIH HHS · GM28454 · United States
External Links
PubMed source DOI: 10.1128/mr.54.1.52-65.1990 PMC Full Text
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