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PMID: 2181275 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

HPR1, a novel yeast gene that prevents intrachromosomal excision recombination, shows carboxy-terminal homology to the Saccharomyces cerevisiae TOP1 gene.

Molecular and cellular biology ·Vol. 10 ·No. 4 ·1990-04-00 ·Pages 1439-51

Aguilera A, Klein HL

Abstract

The HPR1 gene has been cloned by complementation of the hyperrecombination phenotype of hpr1-1 strains by using a color assay system. HPR1 is a gene that is in single copy on chromosome IV of Saccharomyces cerevisiae, closely linked to ARO1, and it codes for a putative protein of 752 amino acids (molecular mass, 88 kilodaltons). Computer searches revealed homology (48.8% conserved homology; 24.8% identity) with the S. cerevisiae TOP1 gene in an alpha-helical stretch of 129 amino acids near the carboxy-terminal region of both proteins. The ethyl methanesulfonate-induced hpr1-1 mutation is a single-base change that produces a stop codon at amino acid 559 coding for a protein that lacks the carboxy-terminal TOP1 homologous region. Haploid strains carrying deletions of the HPR1 gene show a slightly reduced mitotic growth rate and extremely high rates of intrachromosomal excision recombination (frequency, 10 to 15%) but have a undetectable effect on rDNA recombination. Double-null mutants hpr1 top1 grow very poorly. We conclude that Hpr1 is a novel eucaryotic protein, mutation of which causes an increase in mitotic intrachromosomal excision recombination, and that it may be functionally related to an activity of the topoisomerase I protein.

MeSH Terms
Alleles Amino Acid Sequence Base Sequence Chromosomes, Fungal Cloning, Molecular DNA, Fungal/genetics DNA, Ribosomal/genetics Fungal Proteins/genetics Genes, Fungal Genetic Complementation Test Genotype Molecular Sequence Data Mutation Nuclear Proteins Plasmids RNA, Fungal/genetics Recombination, Genetic Restriction Mapping Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Homology, Nucleic Acid
Chemicals
DNA, Fungal DNA, Ribosomal Fungal Proteins HPR1 protein, S cerevisiae Nuclear Proteins RNA, Fungal Saccharomyces cerevisiae Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Aguilera A
Department of Biochemistry, New York University Medical Center, New York 10016.
Klein H L
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.4.1439-1451.1990
Published
1990-04-00
Pages
1439-51
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362246
Subset
IM
Grants
NIGMS NIH HHS · GM30439 · United States
Databases
GENBANK
M30484
External Links
PubMed source DOI: 10.1128/mcb.10.4.1439-1451.1990 PMC Full Text View journal details
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