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PMID: 2182533 Published · ppublish English Journal Article

Recombinant human granulocyte-macrophage colony-stimulating factor induces granule exocytosis from human polymorphonuclear neutrophils.

Inflammation ·Vol. 14 ·No. 1 ·1990-02-00 ·Pages 83-92

Smith RJ, Justen JM, Sam LM

Abstract

Exposure of human polymorphonuclear neutrophils (PMNs) to recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) resulted in a time- and concentration-dependent (3-100 units/ml) extracellular release of a specific (vitamin B12-binding protein) but not azurophil granule constituent (myeloperoxidase). Negligible granule exocytosis occurred if PMNs were not preincubated with cytochalasin B prior to contact with GM-CSF. The extent of degranulation elicited with GM-CSF was reduced but not abolished when PMNs were incubated with EGTA in calcium-free medium. GM-CSF did not stimulate a rise in the cytosolic-free calcium concentration ([Ca2+]i), and it had no effect on PMN protein kinase C (PKC) activity.

MeSH Terms
Calcium/physiology Cell Degranulation/drug effects Colony-Stimulating Factors/pharmacology Cytochalasin B/pharmacology Exocytosis/drug effects Granulocyte-Macrophage Colony-Stimulating Factor Growth Substances/pharmacology Humans In Vitro Techniques Neutrophils/drug effects,enzymology,ultrastructure Protein Kinase C/blood,isolation & purification Recombinant Proteins/pharmacology
Chemicals
Colony-Stimulating Factors Growth Substances Recombinant Proteins Cytochalasin B Granulocyte-Macrophage Colony-Stimulating Factor Protein Kinase C Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Smith R J
Department of Hypersensitivity Diseases Research, Upjohn Company, Kalamazoo, Michigan 49008.
Justen J M
Sam L M
References (43)
43 references, click to expand
  1. Intracellular control of human neutrophil secretion. I. C5a-induced stimulus-specific desensitization and the effects of cytochalasin B.
    J Immunol. 1978 Sep;121(3):851-5 PMID: 211165
  2. Inositol trisphosphate and diacylglycerol: two interacting second messengers.
    Annu Rev Biochem. 1987;56:159-93 PMID: 3304132
  3. Neutrophil degranulation: evidence pertaining to its mediation by the combined effects of leukotriene B4, platelet-activating factor, and 5-HETE.
    J Cell Physiol. 1985 Feb;122(2):229-39 PMID: 2981891
  4. Human granulocyte-macrophage colony-stimulating factor is a neutrophil activator.
    Nature. 1985 Mar 28-Apr 3;314(6009):361-3 PMID: 2984574
  5. Human neutrophils contain an intracellular pool of putative receptors for the chemoattractant N-formyl-methionyl-leucyl-phenylalanine.
    Blood. 1983 Oct;62(4):792-9 PMID: 6309284
  6. Characterization of formylmethionyl-leucyl-phenylalanine stimulation of inositol trisphosphate accumulation in rabbit neutrophils.
    Mol Pharmacol. 1985 Jan;27(1):74-8 PMID: 2981403
  7. High-affinity binding of granulocyte-macrophage colony-stimulating factor to normal and leukemic human myeloid cells.
    Proc Natl Acad Sci U S A. 1986 Feb;83(3):669-73 PMID: 3484823
  8. Effects of recombinant human granulocyte and macrophage colony-stimulating factors on signal transduction pathways in human granulocytes.
    J Immunol. 1987 Nov 15;139(10):3422-30 PMID: 3119708
  9. Role of a guanine nucleotide regulatory protein in the activation of phospholipase C by different chemoattractants.
    J Immunol. 1987 Jun 15;138(12):4374-80 PMID: 3108387
  10. Leukocyte adhesion receptors are stored in peroxidase-negative granules of human neutrophils.
    J Exp Med. 1987 Dec 1;166(6):1641-53 PMID: 2824654
  11. Human granulocyte-macrophage colony-stimulating factor and other cytokines prime human neutrophils for enhanced arachidonic acid release and leukotriene B4 synthesis.
    J Immunol. 1988 Jun 15;140(12):4315-22 PMID: 2453577
  12. Involvement of guanine nucleotide binding proteins in neutrophil activation and priming by GM-CSF.
    Blood. 1989 Feb;73(2):588-91 PMID: 2537115
  13. Characteristics of 1-O-hexadecyl- and 1-O-octadecyl-2-O-acetyl-sn-glyceryl-3-phosphorylcholine-stimulated granule enzyme release from human neutrophils.
    Clin Immunol Immunopathol. 1983 Jul;28(1):13-28 PMID: 6872358
  14. Separation and characterization of human neutrophil granules.
    Am J Pathol. 1974 Oct;77(1):41-66 PMID: 4447123
  15. Human GM-CSF primes neutrophils for enhanced oxidative metabolism in response to the major physiological chemoattractants.
    Blood. 1987 Jan;69(1):18-21 PMID: 3024757
  16. Biosynthetic human GM-CSF modulates the number and affinity of neutrophil f-Met-Leu-Phe receptors.
    J Immunol. 1986 Dec 1;137(11):3584-7 PMID: 3491142
  17. Inositol trisphosphate, a novel second messenger in cellular signal transduction.
    Nature. 1984 Nov 22-28;312(5992):315-21 PMID: 6095092
  18. Platelet-activating factor as a stimulus of exocytosis in human neutrophils.
    Biochim Biophys Acta. 1986 Aug 29;888(1):42-8 PMID: 3017443
  19. Nonsteroid anti-inflammatory agents: regulators of the phagocytic secretion of lysosomal enzymes from guinea-pig neutrophils.
    J Pharmacol Exp Ther. 1978 Nov;207(2):618-29 PMID: 712644
  20. Microfilaments in cellular and developmental processes.
    Science. 1971 Jan 15;171(3967):135-43 PMID: 5538822
  21. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.
    J Biol Chem. 1982 Jul 10;257(13):7847-51 PMID: 7085651
  22. Cytochalasin B enhancement of the diacylglycerol response in formyl peptide-stimulated neutrophils.
    J Biol Chem. 1986 Dec 5;261(34):15900-5 PMID: 3782096
  23. Stimulus response coupling in the human neutrophil. I. Kinetic analysis of changes in calcium permeability.
    J Biol Chem. 1984 Apr 10;259(7):4070-5 PMID: 6323454
  24. Platelet-activating factor production in human neutrophils by sequential stimulation with granulocyte-macrophage colony-stimulating factor and the chemotactic factors C5A or formyl-methionyl-leucyl-phenylalanine.
    J Immunol. 1989 May 1;142(9):3213-8 PMID: 2540238
  25. RAPID CHARCOAL ASSAY FOR INTRINSIC FACTOR (IF), GASTRIC JUICE UNSATURATED B12 BINDING CAPACITY, ANTIBODY TO IF, AND SERUM UNSATURATED B12 BINDING CAPACITY.
    Blood. 1965 Jun;25:875-84 PMID: 14292869
  26. Activation of the human neutrophil secretory process with 5(S),12(R)-dihydroxy-6,14-cis-8,10-trans-eicosatetraenoic acid.
    Inflammation. 1984 Dec;8(4):365-84 PMID: 6097546
  27. Quantitative analysis of the cytosolic free calcium dependency of exocytosis from three subcellular compartments in intact human neutrophils.
    J Cell Biol. 1986 Jun;102(6):2197-204 PMID: 3011810
  28. Hormonal control of lysosomal enzyme release from human neutrophils. Effects of autonomic agents on enzyme release, phagocytosis, and cylic nucleotide levels.
    J Exp Med. 1974 Jun 1;139(6):1395-414 PMID: 4364334
  29. Leukotriene production in human neutrophils primed by recombinant human granulocyte/macrophage colony-stimulating factor and stimulated with the complement component C5A and FMLP as second signals.
    J Exp Med. 1988 Apr 1;167(4):1281-95 PMID: 2833556
  30. Recombinant human granulocyte-macrophage colony-stimulating factor stimulates in vitro mature human neutrophil and eosinophil function, surface receptor expression, and survival.
    J Clin Invest. 1986 Nov;78(5):1220-8 PMID: 3021817
  31. Stimulation of guanylate cyclase activity and reduction of adenylate cyclase activity by granulocyte-macrophage colony-stimulating factor in human blood neutrophils.
    J Immunol. 1988 Apr 15;140(8):2695-701 PMID: 2895792
  32. Mechanisms of lysosomal enzyme release from human leukocytes. II. Effects of cAMP and cGMP, autonomic agonists, and agents which affect microtubule function.
    J Clin Invest. 1974 Jan;53(1):297-309 PMID: 4357615
  33. The role of protein kinase C in cell surface signal transduction and tumour promotion.
    Nature. 1984 Apr 19-25;308(5961):693-8 PMID: 6232463
  34. The human hematopoietic colony-stimulating factors.
    Science. 1987 Jun 5;236(4806):1229-37 PMID: 3296190
  35. Function and stimulus-specific effects of phorbol 12-myristate 13-acetate on human polymorphonuclear neutrophils: autoregulatory role for protein kinase C in signal transduction.
    Inflammation. 1988 Dec;12(6):597-611 PMID: 2851553
  36. Release of gelatinase from a novel secretory compartment of human neutrophils.
    J Clin Invest. 1982 Sep;70(3):518-25 PMID: 6286726
  37. Characterization of the human granulocyte-macrophage colony-stimulating factor receptor.
    J Biol Chem. 1988 Feb 5;263(4):1834-41 PMID: 2828352
  38. Granulocyte-macrophage colony-stimulating factor induces the expression of the CD11b surface adhesion molecule on human granulocytes in vivo.
    Blood. 1988 Aug;72(2):691-7 PMID: 3042045
  39. Effect of tumor necrosis factor and granulocyte/macrophage colony-stimulating factor on neutrophil degranulation.
    J Immunol. 1989 May 1;142(9):3199-205 PMID: 2651522
  40. Cytokines in chronic inflammatory arthritis. II. Granulocyte-macrophage colony-stimulating factor in rheumatoid synovial effusions.
    J Clin Invest. 1989 Mar;83(3):876-82 PMID: 2646320
  41. Studies on guanylate cyclase. A new assay method for guanylate cyclase and properties of the cyclase from rat brain.
    J Biol Chem. 1974 Jul 10;249(13):4207-11 PMID: 4153082
  42. The differential mobilization of human neutrophil granules. Effects of phorbol myristate acetate and ionophore A23187.
    Am J Pathol. 1977 May;87(2):273-84 PMID: 322507
  43. Characteristics of N-formyl-methionyl-leucyl-phenylalanine as an inducer of lysosomal enzyme release from human neutrophils.
    Inflammation. 1980 Mar;4(1):73-88 PMID: 7390615
Article Info
Journal
Inflammation
Abbr.
Inflammation
ISSN
0360-3997
DOI
10.1007/BF00914032
Published
1990-02-00
Pages
83-92
Language
English
Region
United States
NLM ID
7600105
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00914032 View journal details
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