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PMID: 2183027 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A novel mammalian protein kinase gene (mak) is highly expressed in testicular germ cells at and after meiosis.

Molecular and cellular biology ·Vol. 10 ·No. 5 ·1990-05-00 ·Pages 2261-8

Matsushime H, Jinno A, Takagi N, Shibuya M

Abstract

We isolated a novel gene designated mak (male germ cell-associated kinase) by using weak cross-hybridization with a tyrosine kinase gene (v-ros). Sequence analysis of the cDNA corresponding to the 2.6-kilobase transcript revealed that the predicted product of rat mak consisted of 622 amino acids and contained protein kinase consensus motifs in its amino-terminal region. Comparison of the deduced amino acid sequence of mak in the kinase domain with those of other protein kinase genes demonstrated that mak was approximately 40% identical to the cdc2-CDC28 gene family in Schizosaccharomyces pombe, Saccharomyces cerevisiae, and humans but less identical to most other protein kinase gene products. Expression of mak was highly tissue specific, and its transcripts were detected almost exclusively in testicular cells entering and after meiosis but hardly detectable in ovarian cells including oocytes, after the dictyotene stage. These results suggest that the mak gene plays an important role in spermatogenesis.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Western Cloning, Molecular DNA/genetics Female Male Meiosis Mice Molecular Sequence Data Ovary/physiology Protein Kinases/genetics Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-abl Proto-Oncogenes Rats Spermatogenesis Testis/physiology Tissue Distribution
Chemicals
Proto-Oncogene Proteins DNA Protein Kinases Proto-Oncogene Proteins c-abl
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Matsushime H
Department of Genetics, University of Tokyo, Japan.
Jinno A
Takagi N
Shibuya M
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48 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.5.2261-2268.1990
Published
1990-05-00
Pages
2261-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360573
Subset
IM
Databases
GENBANK
M35862, M35863
External Links
PubMed source DOI: 10.1128/mcb.10.5.2261-2268.1990 PMC Full Text View journal details
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