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PMID: 2184030 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The rhp6+ gene of Schizosaccharomyces pombe: a structural and functional homolog of the RAD6 gene from the distantly related yeast Saccharomyces cerevisiae.

The EMBO journal ·Vol. 9 ·No. 5 ·1990-05-00 ·Pages 1423-30

Reynolds P, Koken MH, Hoeijmakers JH, Prakash S, Prakash L

Abstract

The RAD6 gene of Saccharomyces cerevisiae encodes a ubiquitin conjugating enzyme and is required for DNA repair, DNA-damage-induced mutagenesis and sporulation. Here, we show that RAD6 and the rhp6+ gene from the distantly related yeast Schizosaccharomyces pombe share a high degree of structural and functional homology. The predominantly acidic carboxyl-terminal 21 amino acids present in the RAD6 protein are absent in the rhp6(+)-encoded protein; otherwise, the two proteins are very similar, with 77% identical residues. Like rad6, null mutations of the rhp6+ gene confer a defect in DNA repair, UV mutagenesis and sporulation, and the RAD6 and rhp6+ genes can functionally substitute for one another. These observations suggest that functional interactions between RAD6 (rhp6+) protein and other components of the DNA repair complex have been conserved among eukaryotes.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA Repair/genetics DNA, Fungal/genetics Fungal Proteins/genetics Genetic Complementation Test Ligases/genetics Molecular Sequence Data Mutation Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Saccharomycetales/genetics Schizosaccharomyces/genetics Schizosaccharomyces pombe Proteins Sequence Homology, Nucleic Acid Spores, Fungal/genetics Ubiquitin-Conjugating Enzymes Ultraviolet Rays
Chemicals
DNA, Fungal Fungal Proteins Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins RAD6 protein, S cerevisiae Ubiquitin-Conjugating Enzymes rhp6 protein, S pombe Ligases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Reynolds P
Department of Biophysics, University of Rochester School of Medicine, NY 14642.
Koken M H
Hoeijmakers J H
Prakash S
Prakash L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-05-00
Pages
1423-30
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551830
Subset
IM
Grants
NCI NIH HHS · CA41261 · United States
NIGMS NIH HHS · GM19261 · United States
External Links
PubMed source PMC Full Text View journal details
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