Poly(dA).poly(dT) rich sequences are not sufficient to exclude nucleosome formation in a constitutive yeast promoter.
It was suggested that poly(dA).poly(dT) rich sequences in yeast Saccharomyces cerevisiae act as elements of constitutive promoters by exclusion of nucleosomes (Struhl, K. (1985). Proc. Natl. Acad. Sci. USA 82, 8419-8423). We have mapped the chromatin structure of the pet56-his3-ded1 region in minichromosomes and show that the poly(dA).poly(dT) sequences are located in nuclease sensitive regions. DNA fragments from the nuclease sensitive promoter region of DED1 were used for nucleosome reconstitution in vitro. We show that all sequences can form nucleosome cores and that the poly(dA).poly(dT) sequence can be incorporated in nucleosome cores. The results suggest that the nuclease sensitivity found in vivo is not established by poly(dA).poly(dT) mediated exclusion of nucleosomes.
28 references, click to expand
-
Activation of yeast RNA polymerase II transcription by a thymidine-rich upstream element in vitro.Proc Natl Acad Sci U S A. 1989 Jan;86(2):486-90 PMID: 2643115
-
Chromatin folding modulates nucleosome positioning in yeast minichromosomes.Cell. 1988 Dec 23;55(6):945-53 PMID: 3060264
-
Artificial nucleosome positioning sequences.Proc Natl Acad Sci U S A. 1989 Oct;86(19):7418-22 PMID: 2798415
-
Kinetic analysis of deoxyribonuclease I cleavages in the nucleosome core: evidence for a DNA superhelix.J Mol Biol. 1978 Sep 15;124(2):391-420 PMID: 568667
-
Cromatin and core particles formed from the inner histones and synthetic polydeoxyribonucleotides of defined sequence.Nucleic Acids Res. 1979 Apr;6(4):1387-415 PMID: 450700
-
Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructures of chromatin.J Cell Biol. 1979 Nov;83(2 Pt 1):403-27 PMID: 387806
-
Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene.Gene. 1980 Jul;10(2):157-66 PMID: 6248420
-
Stability of the higher-order structure of chicken-erythrocyte chromatin in solution.Eur J Biochem. 1981 Oct;119(3):469-76 PMID: 7308195
-
Nucleosomes will not form on double-stranded RNa or over poly(dA).poly(dT) tracts in recombinant DNA.Nucleic Acids Res. 1981 Dec 21;9(24):6869-88 PMID: 7335494
-
Structural features of a phased nucleosome core particle.Proc Natl Acad Sci U S A. 1983 Jan;80(1):51-5 PMID: 6572008
-
Promoter elements, regulatory elements, and chromatin structure of the yeast his3 gene.Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:901-10 PMID: 6305590
-
Nucleosome reconstitution on plasmid-inserted poly(dA) . poly(dT).EMBO J. 1982;1(2):173-9 PMID: 6325153
-
Nuclease digestion of circular TRP1ARS1 chromatin reveals positioned nucleosomes separated by nuclease-sensitive regions.J Mol Biol. 1984 Aug 25;177(4):715-33 PMID: 6384525
-
Local protein-DNA interactions may determine nucleosome positions on yeast plasmids.Nature. 1985 May 16-22;315(6016):250-2 PMID: 3889654
-
Nucleotide sequence and transcriptional mapping of the yeast pet56-his3-ded1 gene region.Nucleic Acids Res. 1985 Dec 9;13(23):8587-601 PMID: 3001645
-
Naturally occurring poly(dA-dT) sequences are upstream promoter elements for constitutive transcription in yeast.Proc Natl Acad Sci U S A. 1985 Dec;82(24):8419-23 PMID: 3909145
-
DNA bending and its relation to nucleosome positioning.J Mol Biol. 1985 Dec 20;186(4):773-90 PMID: 3912515
-
Suppressors of Saccharomyces cerevisiae his3 promoter mutations lacking the upstream element.Mol Cell Biol. 1985 Aug;5(8):1901-9 PMID: 3018536
-
Nucleosome positioning in vivo and in vitro.Bioessays. 1986 Apr;4(4):172-6 PMID: 3539111
-
Protein-DNA interactions and nuclease-sensitive regions determine nucleosome positions on yeast plasmid chromatin.J Mol Biol. 1986 Jul 20;190(2):177-90 PMID: 3540310
-
Sequence periodicities in chicken nucleosome core DNA.J Mol Biol. 1986 Oct 20;191(4):659-75 PMID: 3806678
-
Core particle, fiber, and transcriptionally active chromatin structure.Annu Rev Cell Biol. 1986;2:117-47 PMID: 3548764
-
A bacteriophage RNA polymerase transcribes in vitro through a nucleosome core without displacing it.Cell. 1987 Aug 28;50(5):801-8 PMID: 3621345
-
Distinguishing between mechanisms of eukaryotic transcriptional activation with bacteriophage T7 RNA polymerase.Cell. 1987 Sep 25;50(7):1047-55 PMID: 3304661
-
The structure of an oligo(dA).oligo(dT) tract and its biological implications.Nature. 1987 Nov 19-25;330(6145):221-6 PMID: 3670410
-
Two DNA-binding factors recognize specific sequences at silencers, upstream activating sequences, autonomously replicating sequences, and telomeres in Saccharomyces cerevisiae.Mol Cell Biol. 1988 Jan;8(1):210-25 PMID: 3275867
-
Nuclease hypersensitive sites in chromatin.Annu Rev Biochem. 1988;57:159-97 PMID: 3052270
-
A DNA binding protein that recognizes oligo(dA).oligo(dT) tracts.EMBO J. 1989 Jun;8(6):1867-77 PMID: 2670564
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
