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PMID: 21943598 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

A resource of Cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex.

Neuron ·Vol. 71 ·No. 6 ·2011-09-22 ·Pages 995-1013

Taniguchi H, He M, Wu P, Kim S, Paik R, Sugino K, Kvitsiani D, Kvitsani D, Fu Y, Lu J, Lin Y, Miyoshi G, Shima Y, Fishell G, Nelson SB, Huang ZJ

Abstract

A key obstacle to understanding neural circuits in the cerebral cortex is that of unraveling the diversity of GABAergic interneurons. This diversity poses general questions for neural circuit analysis: how are these interneuron cell types generated and assembled into stereotyped local circuits and how do they differentially contribute to circuit operations that underlie cortical functions ranging from perception to cognition? Using genetic engineering in mice, we have generated and characterized approximately 20 Cre and inducible CreER knockin driver lines that reliably target major classes and lineages of GABAergic neurons. More select populations are captured by intersection of Cre and Flp drivers. Genetic targeting allows reliable identification, monitoring, and manipulation of cortical GABAergic neurons, thereby enabling a systematic and comprehensive analysis from cell fate specification, migration, and connectivity, to their functions in network dynamics and behavior. As such, this approach will accelerate the study of GABAergic circuits throughout the mammalian brain.

MeSH Terms
Animals Cell Differentiation/physiology Cell Line Cerebral Cortex/cytology,metabolism Gene Expression Regulation Gene Knock-In Techniques Genes, Reporter Integrases/metabolism Interneurons/cytology,physiology Mice Mice, Transgenic Neurons/cytology,physiology Stem Cells/physiology gamma-Aminobutyric Acid/metabolism
Chemicals
gamma-Aminobutyric Acid Cre recombinase Integrases
Authors & Affiliations
16 authors, click to expand affiliations / ORCID
Taniguchi Hiroki
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
He Miao
Wu Priscilla
Kim Sangyong
Paik Raehum
Sugino Ken
Kvitsiani Duda
Kvitsani Duda
Fu Yu
Lu Jiangteng
Lin Ying
Miyoshi Goichi
Shima Yasuyuki
Fishell Gord
Nelson Sacha B
Huang Z Josh
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Article Info
Journal
Neuron
Abbr.
Neuron
ISSN
1097-4199
DOI
10.1016/j.neuron.2011.07.026
Published
2011-09-22
Epub
2011-00-21
Pages
995-1013
Language
English
Region
United States
NLM ID
8809320
PMCID
PMC3779648
Subset
IM
Grants
NIMH NIH HHS · U01 MH078844 · United States
NIMH NIH HHS · U01 MH078844-03 · United States
NIMH NIH HHS · U01 MH078844-01 · United States
NIMH NIH HHS · U01 MH078844-02 · United States
Corrections
ErratumIn
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External Links
PubMed source DOI: 10.1016/j.neuron.2011.07.026 PMC Full Text View journal details
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