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PMID: 2201253 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence analysis and evolutionary perspectives of ROB-1 beta-lactamase.

Antimicrobial agents and chemotherapy ·Vol. 34 ·No. 7 ·1990-07-00 ·Pages 1354-9

Juteau JM, Levesque RC

Abstract

The nucleotide sequence of the ROB-1 beta-lactamase gene from Haemophilus influenzae plasmid RRob was determined. The structural gene encodes a polypeptide of 305 amino acids, with an estimated molecular mass of 30,424 for the mature form of the protein. The ROB-1 gene showed low homologies with other beta-lactamases at the nucleic acid level. By using two statistical computer methods, assessment of the extent of similarity between ROB-1 and other known beta-lactamase amino acid sequences suggested that ROB-1 is a class A enzyme. Alignment of class A beta-lactamases with ROB-1 identified conserved residues. The use of a mutation matrix for detecting distance relationships indicated that ROB-1 has higher values and homologies with beta-lactamases of gram-positive bacteria, giving insight into its ancestry and divergence.

MeSH Terms
Amino Acid Sequence Base Sequence Biological Evolution Cloning, Molecular Computers DNA, Bacterial/analysis Escherichia coli/genetics Genes Genes, Bacterial Molecular Sequence Data Nucleotides/analysis Plasmids beta-Lactamases/genetics
Chemicals
DNA, Bacterial Nucleotides beta-Lactamases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Juteau J M
Département de Microbiologie, Faculté de Médecine, Université Laval, Quebec, Canada.
Levesque R C
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.34.7.1354
Published
1990-07-00
Pages
1354-9
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC175980
Subset
IM
Databases
GENBANK
X13597
External Links
PubMed source DOI: 10.1128/AAC.34.7.1354 PMC Full Text View journal details
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