Home LiteratureArticle Details
PMID: 2201751 Published · ppublish English Comparative Study Journal Article

Cloning and characterization of cDNAs for murine macrophage inflammatory protein 2 and its human homologues.

The Journal of experimental medicine ·Vol. 172 ·No. 3 ·1990-09-01 ·Pages 911-9

Tekamp-Olson P, Gallegos C, Bauer D, McClain J, Sherry B, Fabre M, van Deventer S, Cerami A

Abstract

A cDNA clone of murine macrophage inflammatory protein 2 (MIP-2) has been isolated from a library prepared from lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and the nucleotide sequence determined. This cDNA was used to clone cDNAs for human homologues of MIP-2 from a library prepared from phorbol myristate acetate-treated and LPS-stimulated U937 cells. Two homologues were isolated and sequenced. Human MIP-2 alpha and MIP-2 beta are highly homologous to each other and to a previously isolated gene, human gro/melanoma growth-stimulating activity (MGSA). These three human genes, MIP-2 alpha, MIP-2 beta, and gro/MGSA, constitute a sub-family within the cytokine family represented by platelet factor 4 and interleukin 8.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Carrier Proteins/genetics Cell Line Chemokine CXCL2 Chemokines, CXC Chemotactic Factors/genetics Cloning, Molecular DNA/genetics,isolation & purification Gene Library Humans Intercellular Signaling Peptides and Proteins Interleukin-8 Mice Molecular Sequence Data Oligonucleotide Probes Sequence Homology, Nucleic Acid
Chemicals
CXCL2 protein, human CXCL3 protein, human Carrier Proteins Chemokine CXCL2 Chemokines, CXC Chemotactic Factors Intercellular Signaling Peptides and Proteins Interleukin-8 Oligonucleotide Probes DNA
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Tekamp-Olson P
Chiron Corporation, Emeryville, California 94608.
Gallegos C
Bauer D
McClain J
Sherry B
Fabre M
van Deventer S
Cerami A
References (53)
53 references, click to expand
  1. Isolation, crystallization, and primary amino acid sequence of human platelet factor 4.
    J Biol Chem. 1977 Sep 25;252(18):6276-9 PMID: 893407
  2. Constitutive expression of a gene encoding a polypeptide homologous to biologically active human platelet protein in Rous sarcoma virus-transformed fibroblasts.
    Proc Natl Acad Sci U S A. 1987 Oct;84(19):6715-9 PMID: 2821543
  3. Constitutive overexpression of a growth-regulated gene in transformed Chinese hamster and human cells.
    Proc Natl Acad Sci U S A. 1987 Oct;84(20):7188-92 PMID: 2890161
  4. Purification and partial biochemical characterization of a human monocyte-derived, neutrophil-activating peptide that lacks interleukin 1 activity.
    J Immunol. 1987 Nov 15;139(10):3474-83 PMID: 2824608
  5. Purification of a human monocyte-derived neutrophil chemotactic factor that has peptide sequence similarity to other host defense cytokines.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):9233-7 PMID: 3480540
  6. Purification and amino acid sequencing of NAF, a novel neutrophil-activating factor produced by monocytes.
    Biochem Biophys Res Commun. 1987 Dec 16;149(2):755-61 PMID: 3322281
  7. Optimal alignments in linear space.
    Comput Appl Biosci. 1988 Mar;4(1):11-7 PMID: 3382986
  8. Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor.
    J Exp Med. 1988 Jun 1;167(6):1883-93 PMID: 3260265
  9. Cloning and characterization of a cDNA for murine macrophage inflammatory protein (MIP), a novel monokine with inflammatory and chemokinetic properties.
    J Exp Med. 1988 Jun 1;167(6):1939-44 PMID: 3290382
  10. Molecular characterization and chromosomal mapping of melanoma growth stimulatory activity, a growth factor structurally related to beta-thromboglobulin.
    EMBO J. 1988 Jul;7(7):2025-33 PMID: 2970963
  11. Functional diversity of gro gene expression in human fibroblasts and mammary epithelial cells.
    Proc Natl Acad Sci U S A. 1988 Dec;85(24):9645-9 PMID: 3264403
  12. Identification and characterization of macrophage inflammatory protein 2.
    Proc Natl Acad Sci U S A. 1989 Jan;86(2):612-6 PMID: 2643119
  13. A general method applicable to the search for similarities in the amino acid sequence of two proteins.
    J Mol Biol. 1970 Mar;48(3):443-53 PMID: 5420325
  14. Establishment and characterization of a human histiocytic lymphoma cell line (U-937).
    Int J Cancer. 1976 May 15;17(5):565-77 PMID: 178611
  15. Amino acid sequence of human platelet factor 4.
    Proc Natl Acad Sci U S A. 1977 Jun;74(6):2256-8 PMID: 267922
  16. A method for isolation of intact, translationally active ribonucleic acid.
    DNA. 1983;2(4):329-35 PMID: 6198133
  17. How signal sequences maintain cleavage specificity.
    J Mol Biol. 1984 Feb 25;173(2):243-51 PMID: 6423828
  18. Rapid and sensitive protein similarity searches.
    Science. 1985 Mar 22;227(4693):1435-41 PMID: 2983426
  19. Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.
    Proc Natl Acad Sci U S A. 1985 Mar;82(6):1585-8 PMID: 3856838
  20. The platelet-derived growth factor-inducible KC gene encodes a secretory protein related to platelet alpha-granule proteins.
    J Biol Chem. 1989 Mar 5;264(7):4133-7 PMID: 2917992
  21. Inflammatory cytokines induce synthesis and secretion of gro protein and a neutrophil chemotactic factor but not beta 2-microglobulin in human synovial cells and fibroblasts.
    Biochem J. 1989 Apr 15;259(2):585-8 PMID: 2655583
  22. Identification and characterization of PF4varl, a human gene variant of platelet factor 4.
    Mol Cell Biol. 1989 Apr;9(4):1445-51 PMID: 2725510
  23. Expression and secretion of gro/MGSA by stimulated human endothelial cells.
    EMBO J. 1989 Jun;8(6):1761-6 PMID: 2670560
  24. The three-dimensional structure of bovine platelet factor 4 at 3.0-A resolution.
    J Biol Chem. 1989 Feb 5;264(4):2092-9 PMID: 2914894
  25. Determination of the secondary structure of interleukin-8 by nuclear magnetic resonance spectroscopy.
    J Biol Chem. 1989 Nov 15;264(32):18907-11 PMID: 2681204
  26. Myelopoietic enhancing effects of murine macrophage inflammatory proteins 1 and 2 on colony formation in vitro by murine and human bone marrow granulocyte/macrophage progenitor cells.
    J Exp Med. 1989 Nov 1;170(5):1583-94 PMID: 2478652
  27. The neutrophil chemoattractant produced by the rat kidney epithelioid cell line NRK-52E is a protein related to the KC/gro protein.
    J Biol Chem. 1989 Nov 25;264(33):19559-63 PMID: 2684956
  28. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8) resides in a gene cluster along with several other members of the platelet factor 4 gene superfamily.
    Hum Genet. 1990 Jan;84(2):185-7 PMID: 1967588
  29. Endotoxin-responsive sequences control cachectin/tumor necrosis factor biosynthesis at the translational level.
    J Exp Med. 1990 Feb 1;171(2):465-75 PMID: 2303781
  30. Three-dimensional structure of interleukin 8 in solution.
    Biochemistry. 1990 Feb 20;29(7):1689-96 PMID: 2184886
  31. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  32. A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides.
    J Mol Biol. 1983 Jun 25;167(2):391-409 PMID: 6345794
  33. Molecular cloning of gene sequences regulated by platelet-derived growth factor.
    Cell. 1983 Jul;33(3):939-47 PMID: 6872001
  34. Transcription termination and 3' processing: the end is in site!
    Cell. 1985 Jun;41(2):349-59 PMID: 2580642
  35. Gamma-interferon transcriptionally regulates an early-response gene containing homology to platelet proteins.
    Nature. 1985 Jun 20-26;315(6021):672-6 PMID: 3925348
  36. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
    Cell. 1986 Jan 31;44(2):283-92 PMID: 3943125
  37. A multiple sequence alignment program.
    Nucleic Acids Res. 1986 Jan 10;14(1):363-74 PMID: 3753772
  38. Identification of a common nucleotide sequence in the 3'-untranslated region of mRNA molecules specifying inflammatory mediators.
    Proc Natl Acad Sci U S A. 1986 Mar;83(6):1670-4 PMID: 2419912
  39. Characterization of human platelet basic protein, a precursor form of low-affinity platelet factor 4 and beta-thromboglobulin.
    Biochemistry. 1986 Apr 22;25(8):1988-96 PMID: 2423119
  40. A new method for predicting signal sequence cleavage sites.
    Nucleic Acids Res. 1986 Jun 11;14(11):4683-90 PMID: 3714490
  41. A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation.
    Cell. 1986 Aug 29;46(5):659-67 PMID: 3488815
  42. Isolation and amino acid sequence of bovine platelet factor 4.
    Arch Biochem Biophys. 1986 Oct;250(1):249-56 PMID: 3767377
  43. Purification of melanoma growth stimulatory activity.
    J Cell Physiol. 1986 Dec;129(3):375-84 PMID: 3465735
  44. Cloning and characterization of platelet factor 4 cDNA derived from a human erythroleukemic cell line.
    Blood. 1987 Jan;69(1):219-23 PMID: 3098319
  45. Secretory products of macrophages.
    J Clin Invest. 1987 Feb;79(2):319-26 PMID: 3543052
  46. Structure of the rat platelet factor 4 gene: a marker for megakaryocyte differentiation.
    Mol Cell Biol. 1987 Feb;7(2):898-904 PMID: 3821732
  47. Transformation by Rous sarcoma virus induces a novel gene with homology to a mitogenic platelet protein.
    Cell. 1987 May 8;49(3):321-8 PMID: 3032449
  48. Interferon-inducible gene maps to a chromosomal band associated with a (4;11) translocation in acute leukemia cells.
    Proc Natl Acad Sci U S A. 1987 May;84(9):2868-71 PMID: 2437586
  49. Induction of mRNA for a serine protease and a beta-thromboglobulin-like protein in mitogen-stimulated human leukocytes.
    J Immunol. 1987 Jul 1;139(1):250-6 PMID: 2953813
  50. Human platelet factor 4 gene is mapped to 4q12----q21.
    Cytogenet Cell Genet. 1987;45(2):67-9 PMID: 3622011
  51. The 3' untranslated region of the human interferon-beta mRNA has an inhibitory effect on translation.
    Proc Natl Acad Sci U S A. 1987 Sep;84(17):6030-4 PMID: 3476924
  52. Cloning large segments of genomic DNA using cosmid vectors.
    Methods Enzymol. 1987;152:199-212 PMID: 2958679
  53. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
    Nucleic Acids Res. 1987 Oct 26;15(20):8125-48 PMID: 3313277
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.172.3.911
Published
1990-09-01
Pages
911-9
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188540
Subset
IM
Databases
GENBANK
X53798, X53799, X53800
External Links
PubMed source DOI: 10.1084/jem.172.3.911 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2