Home LiteratureArticle Details
PMID: 2205580 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Legionella pneumophila htpAB heat shock operon: nucleotide sequence and expression of the 60-kilodalton antigen in L. pneumophila-infected HeLa cells.

Infection and immunity ·Vol. 58 ·No. 10 ·1990-10-00 ·Pages 3380-7

Hoffman PS, Houston L, Butler CA

Abstract

A 60-kilodalton (kDa) immunodominant antigen of Legionella pneumophila is a heat shock protein (HSP) of the GroEL class of HSPs. The gene (htpB) coding the 60-kDa protein was localized to a 3.2-kilobase DNA fragment of L. pneumophila cloned into pUC19 (pSH16) (P. S. Hoffman, C. A. Butler, and F. D. Quinn, Infect. Immun. 57:1731-1739, 1989). The nucleotide sequence of the DNA fragment cloned into M13 confirmed two open reading frames, htpA and htpB, that code for proteins of 96 and 548 amino acids, respectively. A consensus heat shock promoter sequence upstream of the start of htpA was identified, and no obvious promoter sequences were detected upstream of htpB. Amino acid sequence comparison studies revealed that the L. pneumophila HtpB protein exhibited 76% homology with the 65-kDa protein of Mycobacterium tuberculosis and 85% homology with both GroEL of Escherichia coli and HtpB of Coxiella burnetii. A comparison of the amino acid sequences among these proteins revealed several regions of nearly absolute sequence conservation, with the variable regions occurring in common areas. The purified L. pneumophila 60-kDa protein was antigenic for human T lymphocytes. Indirect fluorescent antibody studies indicated that the 60-kDa protein may be located in the periplasm or expressed on the surface by intracellular bacteria, suggesting that a stress-related mechanism may be involved in the expression of this immunodominant antigen.

MeSH Terms
Amino Acid Sequence Antigens, Bacterial/biosynthesis,genetics,immunology Antigens, Surface/biosynthesis,genetics,immunology Base Sequence DNA, Bacterial Fluorescent Antibody Technique HeLa Cells Heat-Shock Proteins/biosynthesis,genetics,immunology,isolation & purification Humans Legionella/genetics Lymphocyte Activation/immunology Molecular Sequence Data Operon Sequence Homology, Nucleic Acid
Chemicals
Antigens, Bacterial Antigens, Surface DNA, Bacterial Heat-Shock Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hoffman P S
Department of Microbiology and Immunology, University of Tennessee, Memphis 38163.
Houston L
Butler C A
References (44)
44 references, click to expand
  1. T cells against a bacterial heat shock protein recognize stressed macrophages.
    Science. 1989 Sep 8;245(4922):1112-5 PMID: 2788923
  2. Protein folding in mitochondria requires complex formation with hsp60 and ATP hydrolysis.
    Nature. 1989 Sep 14;341(6238):125-30 PMID: 2528694
  3. Chlamydial disease pathogenesis. The 57-kD chlamydial hypersensitivity antigen is a stress response protein.
    J Exp Med. 1989 Oct 1;170(4):1271-83 PMID: 2571668
  4. Macrophage-activating T-cell factor(s) produced in an early phase of Legionella pneumophila infection in guinea pigs.
    Infect Immun. 1989 Nov;57(11):3458-65 PMID: 2807531
  5. Three pure chaperone proteins of Escherichia coli--SecB, trigger factor and GroEL--form soluble complexes with precursor proteins in vitro.
    EMBO J. 1989 Sep;8(9):2703-9 PMID: 2531087
  6. Induction of Salmonella stress proteins upon infection of macrophages.
    Science. 1990 May 11;248(4956):730-2 PMID: 1970672
  7. Legionnaires' disease bacterium (Legionella pneumophila) multiples intracellularly in human monocytes.
    J Clin Invest. 1980 Sep;66(3):441-50 PMID: 7190579
  8. Pittsburgh pneumonia agent: direct isolation from human lung tissue.
    J Infect Dis. 1980 Jun;141(6):727-32 PMID: 7391615
  9. Non-specific protection against pulmonary Legionella pneumophila infection in guinea-pigs immunized and challenged with mycobacteria.
    Br J Exp Pathol. 1985 Jun;66(3):333-44 PMID: 3924087
  10. An antigen common to a wide range of bacteria. I. The isolation of a 'common antigen' from Pseudomonas aeruginosa.
    Acta Pathol Microbiol Scand B. 1980 Jun;88(3):143-9 PMID: 6160723
  11. Activated human monocytes inhibit the intracellular multiplication of Legionnaires' disease bacteria.
    J Exp Med. 1981 Nov 1;154(5):1618-35 PMID: 7299350
  12. Cell-mediated immunity in Legionnaires' disease.
    J Clin Invest. 1983 Jun;71(6):1686-97 PMID: 6345589
  13. The Legionnaires' disease bacterium (Legionella pneumophila) inhibits phagosome-lysosome fusion in human monocytes.
    J Exp Med. 1983 Dec 1;158(6):2108-26 PMID: 6644240
  14. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  15. Relative potency of virulent versus avirulent Legionella pneumophila for induction of cell-mediated immunity.
    Infect Immun. 1984 Jun;44(3):753-5 PMID: 6724697
  16. Immunochemical characterization of a protein associated with Mycobacterium leprae cell wall.
    Infect Immun. 1985 Aug;49(2):371-7 PMID: 3894233
  17. Human T-cell clones recognize a major M. leprae protein antigen expressed in E. coli.
    Nature. 1986 Jan 2-8;319(6048):63-6 PMID: 3510397
  18. Immunologic response of patients with legionellosis against major protein-containing antigens of Legionella pneumophila serogroup 1 as shown by immunoblot analysis.
    J Clin Microbiol. 1986 Jan;23(1):92-9 PMID: 3517046
  19. Interferon-gamma-activated human monocytes inhibit the intracellular multiplication of Legionella pneumophila.
    J Immunol. 1986 Oct 15;137(8):2662-9 PMID: 3093580
  20. The 65-kilodalton antigen of Mycobacterium tuberculosis.
    J Bacteriol. 1987 Mar;169(3):1080-8 PMID: 3029018
  21. Guinea pigs sublethally infected with aerosolized Legionella pneumophila develop humoral and cell-mediated immune responses and are protected against lethal aerosol challenge. A model for studying host defense against lung infections caused by intracellular pathogens.
    J Exp Med. 1987 Mar 1;165(3):799-811 PMID: 3819647
  22. Cytolytic activity of human peripheral blood leukocytes against Legionella pneumophila-infected monocytes: characterization of the effector cell and augmentation by interleukin 2.
    J Immunol. 1987 Jul 15;139(2):551-6 PMID: 3496384
  23. Mapping of T cell epitopes using recombinant antigens and synthetic peptides.
    EMBO J. 1987 May;6(5):1245-9 PMID: 2440673
  24. In vivo and in vitro characterization of murine T-cell clones reactive to Mycobacterium tuberculosis.
    Infect Immun. 1987 Sep;55(9):2223-9 PMID: 3114149
  25. Purified 60-kilodalton Legionella protein antigen with Legionella-specific and nonspecific epitopes.
    J Clin Microbiol. 1987 Nov;25(11):2080-4 PMID: 2447117
  26. The Mycobacterium tuberculosis 65-kilodalton antigen is a heat shock protein which corresponds to common antigen and to the Escherichia coli GroEL protein.
    Infect Immun. 1988 Feb;56(2):446-51 PMID: 2892795
  27. Cloning of the mycobacterial epitope recognized by T lymphocytes in adjuvant arthritis.
    Nature. 1988 Jan 14;331(6152):171-3 PMID: 2448638
  28. A heat shock operon in Coxiella burnetti produces a major antigen homologous to a protein in both mycobacteria and Escherichia coli.
    J Bacteriol. 1988 Mar;170(3):1227-34 PMID: 3343219
  29. A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides.
    Nature. 1988 Apr 28;332(6167):800-5 PMID: 3282178
  30. 70K heat shock related proteins stimulate protein translocation into microsomes.
    Nature. 1988 Apr 28;332(6167):805-10 PMID: 3282179
  31. IFN-gamma-activated human alveolar macrophages inhibit the intracellular multiplication of Legionella pneumophila.
    J Immunol. 1988 Jun 1;140(11):3978-81 PMID: 3131422
  32. Homologous plant and bacterial proteins chaperone oligomeric protein assembly.
    Nature. 1988 May 26;333(6171):330-4 PMID: 2897629
  33. Stress proteins are immune targets in leprosy and tuberculosis.
    Proc Natl Acad Sci U S A. 1988 Jun;85(12):4267-70 PMID: 3132709
  34. Immunochemical characterization of and isolation of the gene for a Borrelia burgdorferi immunodominant 60-kilodalton antigen common to a wide range of bacteria.
    Infect Immun. 1988 Aug;56(8):2047-53 PMID: 2840400
  35. A Mycobacterium leprae-specific human T cell epitope cross-reactive with an HLA-DR2 peptide.
    Science. 1988 Oct 14;242(4876):259-61 PMID: 2459778
  36. Heat-shock response in Legionella pneumophila.
    Can J Microbiol. 1988 Oct;34(10):1148-53 PMID: 2904297
  37. Legionnaires disease: historical perspective.
    Clin Microbiol Rev. 1988 Jan;1(1):60-81 PMID: 3060246
  38. Mitogenic stimulation of human B lymphocytes by the mannose-specific adhesin on Escherichia coli type 1 fimbriae.
    J Immunol. 1989 Feb 1;142(3):992-8 PMID: 2563273
  39. Characterization of a Legionella pneumophila extracellular protease exhibiting hemolytic and cytotoxic activities.
    Infect Immun. 1989 Mar;57(3):732-8 PMID: 2917785
  40. The groES and groEL heat shock gene products of Escherichia coli are essential for bacterial growth at all temperatures.
    J Bacteriol. 1989 Mar;171(3):1379-85 PMID: 2563997
  41. Vaccination with the major secretory protein of Legionella pneumophila induces cell-mediated and protective immunity in a guinea pig model of Legionnaires' disease.
    J Exp Med. 1989 Mar 1;169(3):691-705 PMID: 2926324
  42. Cloning and temperature-dependent expression in Escherichia coli of a Legionella pneumophila gene coding for a genus-common 60-kilodalton antigen.
    Infect Immun. 1989 Jun;57(6):1731-9 PMID: 2566581
  43. Peptide binding and release by proteins implicated as catalysts of protein assembly.
    Science. 1989 Jul 28;245(4916):385-90 PMID: 2756425
  44. Immunological characterization of a human homolog of the 65-kilodalton mycobacterial antigen.
    Infect Immun. 1989 Sep;57(9):2786-93 PMID: 2668187
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.58.10.3380-3387.1990
Published
1990-10-00
Pages
3380-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313664
Subset
IM
Grants
NIAID NIH HHS · AI24279 · United States
Databases
GENBANK
M31917, M31918
External Links
PubMed source DOI: 10.1128/iai.58.10.3380-3387.1990 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2