Home LiteratureArticle Details
PMID: 2205583 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Association of rns homologs with colonization factor antigens in clinical Escherichia coli isolates.

Infection and immunity ·Vol. 58 ·No. 10 ·1990-10-00 ·Pages 3442-4

Caron J, Maneval DR, Kaper JB, Scott JR

Abstract

rns is a trans-acting positive regulatory factor required for expression of the colonization factor antigen II (CFA/II) antigens CS1 and CS2 (J. Caron, L. M. Coffield, and J. R. Scott, Proc. Natl. Acad. Sci. USA 86:963-967, 1989). All 35 CFA/II-positive strains hybridized with a rns gene probe, as did all 10 CFA/I strains and all 4 CS4 strains. Hybridization with rns was detected in 25% of non-enterotoxigenic Escherichia coli strains and was not detected in enteric pathogens with low G + C content.

MeSH Terms
Antigens, Bacterial/genetics DNA Probes DNA, Bacterial/analysis,genetics Escherichia coli/classification,genetics Fimbriae Proteins Genes, Bacterial/genetics Humans Nucleic Acid Hybridization Phenotype Plasmids/genetics Restriction Mapping
Chemicals
Antigens, Bacterial DNA Probes DNA, Bacterial colonization factor antigens Fimbriae Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Caron J
Department of Microbiology and Immunology, Emory University Health Sciences Center, Atlanta, Georgia 30322.
Maneval D R
Kaper J B
Scott J R
References (26)
26 references, click to expand
  1. Enterotoxigenic Escherichia coli: identification and characterization.
    J Infect Dis. 1980 Aug;142(2):279-86 PMID: 6106031
  2. Longitudinal studies of infectious diseases and physical growth of children in rural Bangladesh. II. Incidence of diarrhea and association with known pathogens.
    Am J Epidemiol. 1982 Mar;115(3):315-24 PMID: 6278925
  3. Hemagglutination activity and colonization factor antigens I and II in enterotoxigenic and non-enterotoxigenic strains of Escherichia coli isolated from humans.
    Infect Immun. 1982 Apr;36(1):189-97 PMID: 7042570
  4. Identification of enterotoxigenic Escherichia coli by colony hybridization using three enterotoxin gene probes.
    J Infect Dis. 1982 Jun;145(6):863-9 PMID: 7045250
  5. Two mannose-resistant haemagglutinins on enterotoxigenic Escherichia coli of serotype O6:K15:H16 or H-isolated from travellers' and infantile diarrhoea.
    J Gen Microbiol. 1982 Sep;128(9):2081-96 PMID: 6816903
  6. Rapid similarity searches of nucleic acid and protein data banks.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):726-30 PMID: 6572363
  7. Plasmids that code for production of colonization factor antigen II and enterotoxin production in strains of Escherichia coli.
    Infect Immun. 1983 Jun;40(3):1236-9 PMID: 6343243
  8. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  9. An improved colony hybridization method with significantly increased sensitivity for detection of single genes.
    Plasmid. 1983 Nov;10(3):296-8 PMID: 6657780
  10. Expression of plasmids coding for colonization factor antigen II (CFA/II) and enterotoxin production in Escherichia coli.
    J Gen Microbiol. 1983 Dec;129(12):3591-601 PMID: 6142082
  11. Coli surface antigens 1 and 3 of colonization factor antigen II-positive enterotoxigenic Escherichia coli: morphology, purification, and immune responses in humans.
    Infect Immun. 1984 May;44(2):409-20 PMID: 6370866
  12. Detection of an adherence factor of enteropathogenic Escherichia coli with a DNA probe.
    J Infect Dis. 1985 Sep;152(3):560-5 PMID: 2863319
  13. Enzyme-linked immunosorbent assays for the detection of adhesion factor antigens of enterotoxigenic Escherichia coli.
    J Infect Dis. 1985 Dec;152(6):1120-7 PMID: 3934290
  14. Comparison of DNA probes and the Sereny test for identification of invasive Shigella and Escherichia coli strains.
    J Clin Microbiol. 1986 Sep;24(3):498-500 PMID: 3531233
  15. Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent.
    J Infect Dis. 1987 Mar;155(3):377-89 PMID: 3543152
  16. Traditional enteropathogenic Escherichia coli of infantile diarrhea.
    Rev Infect Dis. 1987 Jan-Feb;9(1):28-53 PMID: 3547577
  17. A DNA probe to identify enterohemorrhagic Escherichia coli of O157:H7 and other serotypes that cause hemorrhagic colitis and hemolytic uremic syndrome.
    J Infect Dis. 1987 Jul;156(1):175-82 PMID: 3298451
  18. Genetic control and properties of coli surface antigens of colonization factor antigen IV (PCF8775) of enterotoxigenic Escherichia coli.
    Infect Immun. 1988 Aug;56(8):1974-80 PMID: 2456269
  19. DNA probes for Shiga-like toxins I and II and for toxin-converting bacteriophages.
    J Clin Microbiol. 1988 Jul;26(7):1292-7 PMID: 2842369
  20. Fimbrial structures of enterotoxigenic E. coli.
    Antonie Van Leeuwenhoek. 1988;54(5):395-404 PMID: 2462405
  21. Characterization of CS4 and CS6 antigenic components of PCF8775, a putative colonization factor complex from enterotoxigenic Escherichia coli E8775.
    Infect Immun. 1989 Jan;57(1):164-73 PMID: 2491834
  22. A plasmid-encoded regulatory gene, rns, required for expression of the CS1 and CS2 adhesins of enterotoxigenic Escherichia coli.
    Proc Natl Acad Sci U S A. 1989 Feb;86(3):963-7 PMID: 2563591
  23. A rns-like regulatory gene for colonization factor antigen I (CFA/I) that controls expression of CFA/I pilin.
    Infect Immun. 1990 Apr;58(4):874-8 PMID: 1969396
  24. Expression of CFA/I fimbriae is positively regulated.
    Microb Pathog. 1990 Feb;8(2):91-9 PMID: 1971911
  25. Plasmid-controlled colonization factor associated with virulence in Esherichia coli enterotoxigenic for humans.
    Infect Immun. 1975 Sep;12(3):656-67 PMID: 1100526
  26. Travelers' diarrhea in Mexico. A prospective study of physicians and family members attending a congress.
    N Engl J Med. 1976 Jun 10;294(24):1299-305 PMID: 772435
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.58.10.3442-3444.1990
Published
1990-10-00
Pages
3442-4
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313674
Subset
IM
Grants
NIAID NIH HHS · AI24870 · United States
NIAID NIH HHS · N01AI62528 · United States
External Links
PubMed source DOI: 10.1128/iai.58.10.3442-3444.1990 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2